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User manual NucleoSpin® Plant II Midi / Maxi

  
     
NucleoSpin® Plant II Midi / Maxi

Genomic DNA from plant and fungi

Features
For rapid medium and large scale preparation of genomic DNA from plant cells and fungi
• Two alternative lysis buffers included for optimal processing of various samples and highest flexibility:
   Lysis Buffer PL1, based on CTAB lysis method
   Lysis Buffer PL2, based on SDS lysis method

• NucleoSpin® Filters Midi/Maxi included for clarification of lysate
• Improved buffer compositions – higher yield and purity
• RNase A included
NucleoSpin® Plant II Midi NucleoSpin® Plant II Maxi
Technology Silica-membrane technology Silica-membrane technology
Format Midi spin columns Maxi spin columns
Sample material < 400 mg plant tissue (wet weight)
< 80 mg plant tissue (dry weight)
< 1500 mg plant tissue (wet weight)
< 300 mg plant tissue (dry weight)
Lysate clarification NucleoSpin® Filters Midi NucleoSpin® Filters Maxi
Fragment size 50 bp–approx. 50 kbp 50 bp–approx. 50 kbp
Typical yield 10–100 µg
(400 mg plant tissue, wet weight)
50–300 µg
(1500 mg plant tissue, wet weight)
A260/A280 1.8–1.9 1.8–1.9
Elution volume 200–400 µL 1000–2000 µL
Preparation time 90 min/prep 90 min/prep
Binding capacity 200 µg 500 µg

Applications

• DNA from plant cells and tissue
• DNA from fungi
• Typical downstream applications: PCR, Southern blotting, enzymatic reactions
  
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Ordering information

Product

Preps

Specification REF
NucleoSpin® Plant II Midi

20

NucleoSpin® Plant II Midi Columns with Collection Tubes, NucleoSpin® Filters Midi, Collection Tubes (15 mL), buffers, RNase A

740771.20
NucleoSpin® Plant II Maxi

10

NucleoSpin® Plant II Maxi Columns with Collection Tubes, NucleoSpin® Filters Maxi, Collection Tubes (50 mL), buffers, RNase A

740772.10

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