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NucleoMag® Blood 200 µL

DNA from blood and biological fluids

NucleoMag® Blood 3 mL
Features 
Magnetic-bead based isolation of genomic DNA from whole blood

• One-tube procedure minimizes risk of cross-contamination
• Small elution volumes: > 50 µL (NucleoMag® Blood 200 µL), > 1 mL (NucleoMag® Blood 3 mL)
• Complete processing possible at room temperature
• Easily adapted to automated use
• Consistently high yields

  

NucleoMag® Blood 200 µL

NucleoMag® Blood 3 mL

Technology Magnetic-bead technology
Format Highly reactive superparamagnetic beads
Processing

Manual or automated

Automated*

Sample material
200 µL whole blood
(fresh or frozen, EDTA or citrate treated)
3 mL whole blood
(fresh or frozen, EDTA or citrate treated)
Fragment size 300 bp–approx. 50 kbp 300 bp–approx. 50 kbp
Typical yield 2–8 µg (200 µL blood) 100–130 µg (3 mL blood)
A260/A280 1.6–1.9 1.6–1.9
Elution volume 50–100 µL 1000 µL
Preparation time 40–120 min/ 96 preps* 60 min/24 preps*
Binding capacity 0.4 µg/µL 0.4 µg/µL
Applications**
• Manual or automated isolation of genomic DNA from whole blood
• Typical downstream applications: PCR, real-time PCR, Southern blotting, any kind of enzymatic reaction
*Depending on instrument type, setup, and configuration.
**Kits to be used for research purposes only!

 

  
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Ordering information

Product

Preps

Specification REF
NucleoMag® Blood 200 µL

1 x 96

NucleoMag® B-Beads, buffers, Proteinase K 744501.1

4 x 96

as above 744501.4
NucleoMag® Blood 3 mL

1 x 96

NucleoMag® B-Beads, buffers, Proteinase K 744502.1
Material to be supplied by the user
Square-well Block (separation plate)

4

96-well blocks with 2.1 mL square wells 740481

24

as above 740481.24
E.g., Elution Plate U-bottom (elution plate)

24

96-well microplates with 300 µL u-bottom wells 740486.24

 

For use of NucleoMag® Blood 200 µL only on KingFisher® 96/Flex

 

KingFisher® 96 Accessory Kit B

1 set

Square-well Blocks, Deep-well Tip Combs and Elution Plates for 4 x 96 preparations 744951
Product accessories

Pack of

Specification REF
NucleoMag® SEP

1

magnetic separator 744900

For ordering click here

  
   
  
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