User manual 
Blood L / XL

NucleoSpin® Blood L

Genomic DNA from blood and biological fluids

NucleoSpin® Blood XL
For rapid medium scale purification of DNA from blood
• Ready-to-use DNA in less than 60 min
• Complete removal of PCR inhibitors
• Suitable for whole blood, treated with citrate, EDTA, heparin, CPDA
• Consistently high DNA yields

NucleoSpin® Blood L

NucleoSpin® Blood XL
Technology Silica-membrane technology Silica-membrane technology
Format Midi spin columns* Maxi spin columns*
Sample material 0.2–2 mL whole blood
2 x 107 Human/animal cells
2–10 mL whole blood
108 human/animal cells
Fragment size 200 bp–approx. 50 kbp 200 bp–approx. 50 kbp
Typical yield 40–60 µg (2 mL blood) 200–300 µg (10 mL blood)
A260/A280 1.6–1.9 1.6–1.9
Typical concentration 200–500 ng/µL 200–500 ng/µL
Elution volume 120–200 µL 600–2000 µL
Preparation time 60 min/prep 60 min/prep
Binding capacity 250 µg 700 µg


• DNA from whole blood (human or animal blood, fresh or frozen)
• DNA from whole blood treated with citrate, EDTA, heparin, CPDA
• DNA from serum, plasma, buffy coat, platelets, body fluids (e.g., amniotic fluid)
• DNA from cultured cells
• Typical downstream applications: PCR, Southern blotting, enzymatic reactions
* Centrifugation with a swing-out rotor
** Kits to be used for research purposes only


Ordering information



Specification REF
NucleoSpin® Blood L


NucleoSpin® Blood L Columns with Collection Tubes, Collection Tubes (15 mL), buffers, Proteinase K 740954.20
100 as above 740954.100
NucleoSpin® Blood XL


NucleoSpin® Blood XL Columns with Collection Tubes, Collection Tubes (50 mL), buffers, Proteinase K 740950.10


as above 740950.50

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