User manual 

NucleoSpin® RNA Midi

Supplementary protocol
NucleoSpin® RNA Midi

NucleoSpin® RNA Midi

RNA from cells and tissue

Previously known as NucleoSpin® RNA L
Midi spin kit for the isolation of RNA of highest integrity

• rDNase included for on-column digestion – easy and efficient removal of genomic DNA
• NucleoSpin® Filters Midi (shredders) – efficient sample homogenization and reduction of viscosity
• Up to 600 μg ready-to-use RNA
Technology Silica-membrane technology
Format Midi spin columns
Sample material

< 5 x 107 cultured cells, < 1010 bacterial cells, < 3 x 108 yeast cells,
< 200 mg human / animal tissue

Fragment size > 200 nt
Typical yield

180 µg (107 HeLa cells), 620 µg (4 x 107 HeLa cells)

A260/A280 1.9–2.1
Typical RIN (RNA integrity number) > 9
Elution volume 500–1000 µL
Preparation time 80 min/4 preps
Binding capacity 700 µg
• RNA isolation from cultured cells, tissue (standard protocol)
• RNA from < 1010 bacterial cells (Gram-negative, Gram-positive) or < 3 x 108 yeast cells
• RNA clean-up from reaction mixtures
• RNA from samples stored in RNAlater®
• Typical downstream applications: real-time RT-PCR, Northern blotting, primer extension, 
   array technology, RNase protection assays

* Kits to be used for research purposes only



Ordering information



Specification REF
NucleoSpin® RNA Midi


NucleoSpin® RNA Midi Columns with Collection Tubes
(15 mL), Collection Tubes (15 mL), NucleoSpin® Filters Midi, buffers, RNase-free rDNase

For ordering click here

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