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| NucleoSpin® RNA |
RNA from cells and tissue
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| Features |
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| Mini spin kit for the isolation of RNA of highest integrity |
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 • rDNase included for on-column DNA digestion– easy and efficient removal of contaminating DNA
• NucleoSpin® Filters (shredders) – efficient sample homogenization and reduction of viscosity
• Up to 70 μg ready to use RNA
• Parallel purification of genomic DNA by using the NucleoSpin® RNA/DNA Buffer Set
• Also available for QIAcube® (please see "Ordering information" and contact Technical Service
at techbio@mn-net.com for more information).
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| Technology |
Silica-membrane technology |
| Format |
Mini spin columns |
| Sample material |
< 5 x 106 cultured cells,
< 109 bacterial cells,
< 108 yeast cells,
< 30 mg human / animal tissue
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| Fragment size |
> 200 nt |
| Typical yield |
14 μg (106 HeLa cells), 70 μg (109 bacterial cells)
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| A260/A280 |
1.9–2.1 |
| Typical RIN (RNA integrity number) |
> 9 |
| Elution volume |
40–120 µL |
| Preparation time |
35 min/6 preps |
| Binding capacity |
200 µg |
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Applications*
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• RNA isolation from cultured cells, tissue (standard protocol)
• RNA from < 109 bacterial cells (Gram-negative, Gram-positive) or < 108 yeast cells
• RNA from ≤ 100 μL biological fluids
• RNA clean-up from reaction mixtures
• RNA from samples stored in RNAlater®
• RNA from saliva samples collected with Oragene®•RNA (Genotek)
• Typical downstream applications: real-time RT-PCR, Northern blotting, primer extension,
array technology, RNase protection assays |
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* Kits to be used for research purposes only
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Ordering information
| Product |
Preps
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Specification |
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| NucleoSpin® RNA |
10
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NucleoSpin RNA® Columns with Collection Tubes, Collection Tubes (2 mL), Collection Tubes (1.5 mL), NucleoSpin® Filters, buffers, RNase-free rDNase |
740955.10 |
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50
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as above |
740955.50 |
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250
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as above |
740955.250 |
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240
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Kit for use on QIAcube®*
NucleoSpin RNA® Columns with Collection Tubes, NucleoSpin® Filters, buffers, RNase-free rDNase |
740955.240C |
* NucleoSpin® kits on QIAcube®: The usage of NucleoSpin® kits on the QIAcube® is at your own liability.
MACHEREY-NAGEL is not responsible for loss of warranty claims or other consequences. |
For ordering click here
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