User manual
NucleoSpin® RNA

Supplementary protocols
NucleoSpin® RNA

NucleoSpin® RNA

RNA from cells and tissue

Mini spin kit for the isolation of RNA of highest integrity

• rDNase included for on-column DNA digestion– easy and efficient removal of contaminating DNA
• NucleoSpin® Filters (shredders) – efficient sample homogenization and reduction of viscosity
• Up to 70 μg ready to use RNA
• Parallel purification of genomic DNA by using the NucleoSpin® RNA/DNA Buffer Set

Also available for QIAcube® (please see "Ordering information" and contact Technical Service
techbio@mn-net.com for more information).

Technology Silica-membrane technology
Format Mini spin columns
Sample material

< 5 x 106 cultured cells,
< 109 bacterial cells,
< 108 yeast cells,
< 30 mg human / animal tissue

Fragment size > 200 nt
Typical yield

14 μg (106 HeLa cells), 70 μg (109 bacterial cells)

A260/A280 1.9–2.1
Typical RIN (RNA integrity number) > 9
Elution volume 40–120 µL
Preparation time 35 min/6 preps
Binding capacity 200 µg


• RNA isolation from cultured cells, tissue (standard protocol)
• RNA from < 109 bacterial cells (Gram-negative, Gram-positive) or < 108 yeast cells
• RNA from ≤ 100 μL biological fluids
• RNA clean-up from reaction mixtures
• RNA from samples stored in RNAlater®
• RNA from saliva samples collected with Oragene®RNA (Genotek)
• Typical downstream applications: real-time RT-PCR, Northern blotting, primer extension,
  array technology, RNase protection assays

* Kits to be used for research purposes only



Ordering information



Specification REF
NucleoSpin® RNA


NucleoSpin RNA® Columns with Collection Tubes, Collection Tubes (2 mL), Collection Tubes (1.5 mL), NucleoSpin® Filters, buffers, RNase-free rDNase 740955.10


as above 740955.50


as above 740955.250


Kit for use on QIAcube®*
NucleoSpin RNA® Columns with Collection Tubes, NucleoSpin® Filters, buffers, RNase-free rDNase

* NucleoSpin® kits on QIAcube®: The usage of NucleoSpin® kits on the QIAcube® is at your own liability.
MACHEREY-NAGEL is not responsible for loss of warranty claims or other consequences.

For ordering click here


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