User manual
NucleoBond® HMW DNA

Product flyer
NucleoBond® HMW DNA


NucleoBond® HMW DNA

Genomic DNA from tissue and cells


Large amounts of ultrapure, high molecular weight DNA fragments from diverse samples

• High quality, high molecular weight (HMW) DNA up to 200 kbp
• Minimized DNA shearing due to established anion exchange technology 
• Validated with diverse samples and sequencing platforms

NucleoBond® HMW DNA
Technology Anion exchange chromatography
Format Midi gravity flow columns
Fragment size Up to 200 kbp with > 90% depletion of fragments < 10 kpb
Sample material < 1.5 g plant leaves (ground under liquid nitrogen)
< 107 cultured cells (enzymatic lysis)
< 300 mg solid tissue (cut into small pieces and lysed enzymatically
   with increased lysis time, ground under liquid nitrogen, or lysed by bead beating)
< 30 mg yeast or bacteria (ground under liquid nitrogen)
< 300 mg yeast or bacteria (lysed enzymatically or by bead beating)
< 2 mL liquid sample (e.g., blood, body fluids or enzymatic reactions)

Typical yield

Depending on the sample amount and type
Elution volume 50–250 µL
Processing time 2h/12 preps (including 30 min lysis)

High molecular weight DNA from mammalian tissues, blood samples, invertebrates, plants, bacteria, and yeast

* Kits to be used for research purposes only


Ordering information



Specification REF
NucleoBond® HMW DNA


NucleoBond® HMW Columns with inserted column filters, plastic washers, buffers, Liquid Proteinase K, Liquid RNase  740160.2

20 as above 740160.20
Product accessories

Pack of

Specification REF
NucleoSnap® Finisher Midi


for purification of eluates from NucleoBond® preparations: NucleoSnap® Finisher Columns, buffers, Collection Tubes 740434.10

50 as above 740434.50

NucleoSpin® Finisher Midi


for purification of eluates from NucleoBond® preparations: NucleoSpin® Finisher Columns, buffers, Collection Tubes 740439.10

50 as above 740439.50

MN Bead Tube Holder 1 to house up to 12 bead tubes in combination with a Vortex-Genie® instrument 740469
NucleoSpin® Bead Tubes Type A–G Check www.mn-net.com/beadtubes for more information.
NucleoVac 24 Vacuum Manifold 1 Vacuum manifold for processing NucleoSnap® or NucleoSpin® columns 740299
NucleoVac Mini Adapter 100 Disposable adapters for processing NucleoSpin® or NucleoSnap® columns on the NucleoVac 24 Vacuum Manifold 740297.100
NucleoVac Valves 24 Valves for processing samples with different flow rates on the NucleoVac 24 Vacuum Manifold 740298.24

For ordering click here

Copyright 2019 by MACHEREY-NAGEL GmbH & Co. KG   Imprint / Impressum Privacy StatementTerms Of Use