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NucleoType Blood PCR

DNA amplification from blood samples

 

Features 
Kit for fast genotyping from human and animal blood samples and blood dried on blood cards

• Direct blood handling with the Blood Transfer Tool and DNA preparation within 1 minute
• Inhibitor Removal Pearls for superior PCR results for challenging blood samples
• No step for lysis, disruption, or dilution of blood samples required

Technology Direct HotStart PCR for blood sample material
Format 10 μL PCR (optional up to 50 μL)
Sample material Whole blood from e.g., human, mouse, rat, cat, chicken, rabbit, guinea pig, sheep, or cow treated with EDTA, citrate, or heparin.
Punches from blood storage cards (e.g., NucleoCard® or FTA).
Procedure Pretreatment of blood with 1 Inhibitor Removal Pearl for 50–500 μL blood (optional) and transfer of blood aliquot with the Blood Transfer Tool into PCR mix
PCR Use the NucleoType HotStart PCR Master Mix for fast PCR genotyping.
Amplicon size Recommended for up to 1 kb
Preparation time < 1 min; PCR cycling: 30–90 min (cycler and target size dependent)
Stability 12 months at 4 °C or -20 °C
Applications*

• Direct HotStart PCR analysis from human and animal blood samples

* Kits to be used for research purposes only
  
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Ordering information 

Product

Preps

Specification REF
NucleoType Blood PCR

25

Inhibitor Removal Pearls and Blood Transfer Tool for convenient and rapid blood typing;
NucleoType HotStart PCR Master Mix (2x) containing polymerase, dNTPs, buffer, enhancer, loading dye and stabilizer
743201.25

100

as above 743201.100

500

as above 743201.500
Related product

Pack of

Specification REF
NucleoCard®**

10 

Blood sample storage cards 740403.10

100

see above

* Kits to be used for research purposes only
** NucleoCard® cards are not intended for diagnostic and therapeutic use;
DISTRIBUTION AND USE IN USA IS PROHIBITED FOR PATENT REASONS

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