User manual
NucleoBond® RNA Soil Mini

NucleoBond® RNA Soil Mini

RNA from soil samples

  NucleoSpin® Bead Tubes Type A included

RNA purification from soil samples for qRT-PCR analysis

• Anion exchange technology in Mini format to optimize RNA yield and purity - suitable for qRT-PCR
• Combination of mechanical homogenization and chemical lysis allows processing of large sample
• Parallel preparation of RNA and DNA in one hour

Technology Anion exchange chromatography, gravity flow columns combined with NucleoSpin® Bead Tubes Type A
Format Gravity flow columns
Sample material

0.25–0.5 g soil

Fragment size ³ 100 nt
Typical yield

0.25–2.5 μg

A260/A280 1.5–2.0
Typical RIN (RNA integrity number) > 7.0
Elution volume 50–100 µL
Preparation time 60 min/12 preps
Binding capacity 30 µg


• RNA isolation from soil for qRT-PCR analysis
• Typical downstream applications: real-time RT-PCR

* Kits to be used for research purposes only



Ordering information



Specification REF
NucleoBond® RNA Soil Mini


NucleoBond® RNA Mini Columns, NucleoSpin® Bead Tubes Type A, NucleoSpin® RNA Columns, buffers 740142.10
NucleoBond® RNA Soil Mini 50 as above 740142.50
Related products


Specification REF
DNA Set for NucleoBond® RNA Soil Mini


NucleoSpin® DNA Columns, Collection Tubes, buffers 740143.10
DNA Set for NucleoBond® RNA Soil Mini 50 as above 740143.50
NucleoSpin® Bead Tubes Type A 50 2 mL tubes with 0.6-0.8 mm ceramic beads, for homogenization of soil, sediments, and stool 740786.50
MN Bead Tube Holder 1 to house up to 12 bead tubes in combination with a Vortex-Genie® instrument 740469

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