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NucleoSpin® Gel and PCR Clean-up Midi/Maxi

PCR clean-up / Gel extraction

Binding Buffer NTI –colored pH indicator

Features 
PCR clean-up and gel extraction - the two in one kit

• Two applications in one kit – one buffer with optimal performance for both applications
• High recoveries for fragments down to 50 bp
• Binding buffer with pH indicator
• Separate buffers for single stranded DNA or SDS containing samples available
• Suitable for all gel buffer systems (e.g., TAE, TBE)

NucleoSpin® Gel and PCR Clean-up Midi NucleoSpin® Gel and PCR Clean-up Maxi
Technology Silica membrane technology Silica membrane technology
Format Midi spin columns Maxi spin columns
Sample material

< 4 mL PCR reaction mixture
< 4 g TAE / TBE agarose gel

< 10 mL PCR reaction mixture
< 10 g TAE/TBE agarose gel
Fragment size 50 bp–approx. 20 kbp 50 bp–approx. 20 kbp
Typical recovery 70–95% 70–95%
A260/A280 1.75–1.85 1.75–1.85
Elution volume 200–400 µL 1000 µL
Preparation time 25 min/6 preps 30 min/6 preps
Binding capacity 75 µg 250 µg
Applications

• Purification of PCR products
• Extraction of DNA / RNA from agarose and polyacrylamide gels
• Typical downstream applications: clining, sequencing, PCR, restriction analysis
• NucleoSpin gel and PCR Clean-up ensures complete removal of contaminations auch as:
- nucleoties, primers
- enzymes
- mineral oil
- PCR additives (e.g., saltes betaine, DMSO)
- detergents (e.g., Tween 20, Triton X-100)
- dyes (e.g., ethidiumbromide, crystal violet, Stain G, Midori Green, Roti® Safe GelStain, DNA SafeStain)

 

  
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Ordering information

Product

Preps

Specification REF
NucleoSpin® Gel and PCR Clean-up Midi

20

NucleoSpin® Gel and PCR Clean-up Midi Columns,
buffers
740986.20
NucleoSpin® Gel and PCR Clean-up Maxi 20 NucleoSpin® Gel and PCR Clean-up Maxi Columns,
buffers
740610.20
Product accessories

Pack of

Specification REF
NucleoSpin® Gel and PCR Clean-up 10 NucleoSpin® Gel and PCR Clean-up Columns, Collection Tubes (2 mL), buffers 740609.10
50 as above 740609.50
250 as above 740609.250
Buffer NTB

150 mL

for clean-up of SDS-containing samples 740595.150
Buffer NTC

100 mL

for clean-up of single stranded DNA  740654.100

For ordering click here

  
   
  
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