User manual
NucleoSpin® RNA Stool

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NucleoSpin® RNA Stool

NucleoSpin® RNA Stool

Total RNA from stool

  NucleoSpin® Bead Tubes Type A included

Speedy isolation of total RNA from various stool specimen

• Combination of mechanical homogenization and chemical lysis ensures optimized RNA yield, 
   including small RNA
• Isolated RNA does not show any PCR inhibition due to NucleoSpin® Inhibitor Removal Columns
• Suitable for various human and animal stool samples

Technology Silica membrane technology combined with
NucleoSpin® Bead Tubes Type A
Format Mini spin columns
Sample material

Fresh or frozen stool samples

Fragment size ≥ 18 nt
Typical yield

10–30 µg

A260/A280 1.9–2.1
A260/A230 1.8-2.5
Typical RIN (RNA integrity number) > 7.5
Elution volume 100 µL
Preparation time 70 min/10 preps
Binding capacity 200 µg


• RNA isolation from stool samples
• Typical downstream applications: real-time RT-PCR, Northern blotting, primer extension, array technology,
   RNase protection assays

* Kits to be used for research purposes only



Ordering information



Specification REF
NucleoSpin® RNA Stool


NucleoSpin® RNA Columns with Collection Tubes, NucleoZOL, NucleoSpin® Bead Tubes Type A, NucleoSpin® Inihibitor Removal Columns with Collection Tubes, Collection Tubes (2 mL), Collection Tubes (1.5 mL), rDNase, buffers 740130.10


as above 740130.50


Specification REF
NucleoZOL 200 mL NucleoZOL reagent 740404.200
NucleoSpin® Bead Tubes Type A 50 2 mL tubes with 0.6-0.8 mm ceramic beads, for homogenization of soil, sediments, and stool 740786.50
MN Bead Tube Holder 1 To house up to 12 bead tubes in combination with a Vortex-Genie® instrument 740469

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