Close the lower threaded opening of the NucleoVac 24 Vacuum Manifold with the rubber closing plug (1) . Screw in the tubing connection (2) in the upper opening (3) manually. Do not use a wrench as the conical screw thread might crack if the connection is over-tightened. Attach tube (not included in the product).
For processing NucleoSpin® (4) or NucleoSnap columns with the NucleoVac 24 Vacuum Manifold, insert NucleoSpin® or NucleoSnap columns into the Luer openings (5), (a).
To avoid cross-contamination, it is recommended to use disposable NucleoVac Mini Adapters (6), (b). For processing samples with different flow rates (e.g., large volume samples), it is recommended to use NucleoVac Valves (7) for maintaining constant vacuum pressure throughout the whole procedure (c). Close unused positions with Luer plugs (8), (d).