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NucleoSpin® DNA Stool

Genomic DNA from stool samples

 
Features
Isolation of genomic DNA from stool samples

       

• Suitable for a any stool sample high quality DNA from stool from carnivores,
   omnivores, and herbivores
• NucleoSpin® Bead Tubes for efficient lysis included compatible with the most
   common disruption devices
• NucleoSpin® Inhibitor Removal Columns ensure highest purity of the isolated DNA

Technology Silica-membrane technology combined with NucleoSpin® Bead Tubes Type A
Format Mini spin columns
Sample material

Approx. 200 mg (180–220 mg)* stool samples (fresh or frozen)

Fragment size 200 bp–approx. 50 kbp
Typical yield Depends on sample type, quality, and water content
A260/A280 1.7–1.9
Elution volume 30–100 µL
Preparation time 60 min/10 preps
Binding capacity 50 µg
* For human stool samples approx. 200 mg should be used. For animal stool samples – depending on the
species – a lower amount of sample material may be required for optimal results.
Applications

• Genomic DNA from bacterial and epithelial cells from stool samples
• Typical downstream applications: PCR, real-time PCR, Southern blotting, microarray technology

 

  
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Ordering information

Product

Preps

Specification REF
NucleoSpin® DNA Stool

10

NucleoSpin® DNA Stool Columns, NucleoSpin® Bead Tubes Type A, NucleoSpin® Inhibitor Removal Columns, Collection Tubes (2 mL), Collection Tubes (2 mL, lid), buffers 740472.10

50

as above 740472.50
250 as above 740472.250

 

Product accessories

Pieces

Specification REF
MN Bead Tube Holder 1 Rubber-foam adapter for processing NucleoSpin® Bead Tubes with Vortex-Genie® 2 740469
NucleoSpin® Bead Tubes
Type A
50

2 mL tubes with ceramic beads for sample homogenization
(0.6–0.8 mm ceramic beads; recommended for stool, soil, and sediments)

740786.50
NucleoSpin® Bead Tubes
Type B
50

2 ml tubes with glass beads for sample homogenization
(40–400 μm glass beads; recommended for bacteria)

740812.50
NucleoSpin® Bead Tubes
Type C
50

2 mL tubes with corundum for sample homogenization
(1–3 mm corundum; recommended for yeast and fungi)

740813.50
NucleoSpin® Bead Tubes
Type D
50 2 mL tubes with steel balls for sample homogenization
(3 mm steel balls; recommended for insects, crustaceans, lipid-rich tissue)
740814.50
NucleoSpin® Bead Tubes
Type E
50 2 mL tubes with steel balls and glass beads for sample homogenization
(3 mm steel balls and 40–400 μm glass beads; recommended for bacteria within insects or tissue samples)
740815.50

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