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NucleoMag® DNA FFPE

Application Note
NucleoMag® DNA FFPE

  
     
NucleoMag® DNA FFPE

Genomic DNA from FFPE samples

Features
Magnetic-bead based isolation of genomic DNA from FFPE samples
• Blue colored Paraffin Dissolver (patented technology) for convenient paraffin removal
• Removal of formalin crosslinking by efficient decrosslinking buffer
• High quality DNA for improved performance in downstream applications (e.g., PCR)
• Magnetic-bead technology allows highest flexibility, easy adaption for automated use
Technology Magnetic-bead technology
Format Highly reactive superparamagnetic beads
Processing

Manual or automated

Sample material

≤ 5 mg tissue ≤ 15 mg paraffin

Fragment size 50 bp–approx. 5 kbp
Typical yield Depending on amount and quality of sample
A260/A280 Strongly depending on sample quality
Elution volume > 25 µL
Preparation time 40–120 min/96 preps (excl. lysis)*
Binding capacity 0.4 µg/µL beads

Applications**

• Rapid isolation of DNA from formalin-fixed, paraffin-embedded samples
• Isolation of DNA from fresh and archived FFPE samples
• Isolation of DNA from specimen of object slides
• Typical downstream application: PCR
**Depending on instrument type, setup, and configuration.
**Kits to be used for research purposes only!
  
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Ordering information

Product

Preps

Specification REF
NucleoMag® DNA FFPE

1 x 96

NucleoMag® B-Beads, buffers, Proteinase K 744320.1

4 x 96

as above 744320.4
Material to be supplied by the user
E.g. Rack of Tube Strips (lysis tubes)

4 sets

1 set consists of 1 rack, 12 strips with 8 tubes each, and cap strips 740477
24 sets as above 740477.24
E.g. Square-well Block (separation plate)

4

96-well blocks with 2.1 mL square wells 740481

24

as above 740481.24
E.g. Elution Plate U-bottom (elution plate)

24

96-well microplates with 300 µL u-bottom wells 740486.24

 

For use with KingFisher® 96 platform

 

KingFisher® 96 Accessory Kit A

1 set

Square-well Blocks, Deep-well Tip Combs and Elution Plates for 4 x 96 preparations 744950
Product accessories

Pack of

Specification REF
NucleoMag® SEP

1

magnetic separator 744900
NucleoMag® SEP Mini 1 magnetic separator,
12 x 1.5 mL or 2 mL reaction tubes
744901

For ordering click here

  
     
  
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