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NucleoSpin® 8/96 DNA FFPE

Isolation of genomic DNA from FFPE samples

 
Features
Isolation of total DNA from FFPE samples in flexible 8-well strip or proven 96-well plate Format

• Blue colored Paraffin Dissolver (patented technology) for easy paraffin removal
• Decrosslinking buffer to eliminate formalin crosslinking
• High quality of DNA for improved performance in downstream 
   applications (e.g., PCR)
• Processing under vacuum or by centrifugation, suitable for manual and
   automated processing

NucleoSpin® 8 DNA FFPE NucleoSpin® 96 DNA FFPE
Technology Silica-membrane technology Silica-membrane technology
Format 8-well strips 96-well plates
Processing Manual or automated, vacuum or centrifugation Manual or automated, vacuum or centrifugation
Sample material

≤ 10 mg tissue ≤ 15 mg paraffin

≤ 10 mg tissue ≤ 15 mg paraffin

Fragment size 50 bp–approx. 5 kbp 50 bp–approx. 5 kbp
Typical yield

Depending on amount and quality of sample

Depending on amount and quality of sample

A260/A280 Strongly depending on sample quality Strongly depending on sample quality
Elution volume 100 µL 100 µL
Preparation time 60 min/6 strips (excl. lysis) 60 min/plate (excl. lysis)
Binding capacity 20 µg 20 µg
Applications*
• Rapid isolation of DNA from formalin-fixed, paraffin-embedded samples
• Isolation of DNA from fresh and archived FFPE samples
• Isolation of DNA from specimen of object slides
• Typical downstream application: PCR
*Kits to be used for research purposes only

 

  
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Ordering information

Product

Preps

Specification REF
NucleoSpin® 8 DNA FFPE 12 x 8

NucleoSpin® DNA FFPE Binding Strips, MN Wash Plates, Rack of Tube Strips, Cap Strips, Paraffin Dissolver, buffers, Proteinase K

740242
60 x 8 as above 740242.5
NucleoSpin® 96 DNA FFPE

1 x 96

NucleoSpin® DNA FFPE Binding Plates, MN Wash Plates, Round-well Blocks, MN Square-well Blocks, Rack of Tube Strips, Self-adhering Foil, Paraffin Dissolver, buffers, Proteinase K

740240.1

4 x 96

as above 740240.4
Product accessories Pack of Specification REF
NucleoVac 96 Vacuum Manifold 1 Vacuum manifold for 96-well plates 740681
NucleoVac Vacuum Regulator

1

for controlling of vacuum 740641
Starter Set A 1 for use of NucleoSpin® 8-well strips on the NucleoVac 96 Vacuum Manifold 740682

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