User manual 
NucleoSpin® Blood L Vacuum

Application note
NucleoSpin® Blood L Vacuum

NucleoSpin® Blood L Vacuum

Genomic DNA from blood


DNA purification from up to 2 mL blood using vacuum filtration

• Efficient DNA purification from 2 mL blood samples – complete removal of PCR inhibitors
• Parallel purification of 24 samples in 75 min – special adapter set for
NucleoVac Vacuum Manifold available
• Consistently high DNA yield – suitable for whole blood, treated with EDTA, or citrate 

Technology Silica-membrane technology
Format Midi spin columns
Sample material

1–2 mL whole blood

Fragment size 200 bp–approx. 50 kbp
Typical yield 50–80 µg (2 mL blood)
A260/A280 1.6–1.9
Typical concentration 100–130 ng/µL
Elution volume 2 x 300 µL
Preparation time 75 min/24 preps
Binding capacity 250 µg
• DNA from whole blood (human or animal blood, fresh or frozen)
• DNA from whole blood treated with citrate, EDTA
• Typical downstream applications: PCR, Southern blotting, enzymatic reactions
* Kits to be used for research purposes only


Ordering information



Specification REF
NucleoSpin® L Blood Vacuum


NucleoSpin® Blood L Columns with Collection Tubes, Collection Tubes (1.5 mL), buffers, Proteinase K 740954.24
Product accessories Pack of Specification REF
NucleoVac 96 Vacuum Manifold 1 740681
NucleoVac Vacuum Regulator 1 for controlling of vacuum 740641
Starter Set Midi 1 set

for processing NucleoSpin® Blood L Vacuum under vacuum on NucleoVac 96 Vacuum Manifold or similar manifolds, contains
1 Column Holder Midi, 1 Wash Plate Midi,
1 Elution Tube Holder Midi,
and Dummy Columns


For ordering click here

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