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Protino® 96 Ni-NTA

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Protein Purification Guide

  
     

Protino® 96 Ni-NTA

Purification of His-tag proteins

 Unique Protino® Purification Plate
– leak-free incubations
 
Features
High-throughput purification of His-tag proteins

  
 Efficient protein purification using chelating group NTA (nitrilotriacetic acid)
• Unique Protino® Purification Plate for leak-free incubation during all steps
• Plate outlet and MN Wash Plate prevent cross-contamination
• High well-to-well reproducibility
• High capacity and high affinity
• Purification under native and denaturing conditions
• Processing under vacuum or by centrifugation
Technology

IMAC (immobilized metal ion affinity chromatography)

Chelating ligand NTA (nitrilotriacetic acid)
Format 96-well plates
Matrix

6 % beaded agarose (cross-linked), precharged with Ni2+

Bead size

45–165 µm

Sample volume < 750 µL/well, supplied matrix is sufficient for 96 preps with 50 µL of settled agarose beads per well
Bed volume Variable (50 µL recommended)
Reproducability between wells ± 5 % in yield

Binding capacity*

2 mg per well when using 50 µL of settled agarose
Storage temperature 4–8 °C
* Binding capacity will vary for each polyhistidine-tagged protein and strongly depends on chromatographic conditions
  
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Ordering information

Product

Pack of

Specification REF
Protino® 96 Ni-NTA

1 x 96

Protino® Ni-NTA Agarose, Protino® Purification Plate, MN Wash Plate

745425.1


4 x 96

as above 745425.4
Product accessories

Pack of

Specification REF
Protino® Purification Plate 1 96-well plate, suitable for centrifugation and vacuum 745426.1
4 as above 745426.4
Receiver Plates 10 µm 4 96-well plates with an inserted filter frit of 10 µm pore size, suitable for centrifugation and vacuum 740989.4
NucleoVac 96 Vacuum Manifold

1

740681
NucleoVac Vacuum Regulator

1

for controlling of vacuum 740641
MN Shaker Frame 1 shaking frame for Protino® Purification Plate 740489

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