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NucleoBond® 96 Xtra EF

  
     

NucleoBond® 96 Xtra EF

Transfection-grade, endotoxin-free plasmid DNA

Patented endotoxin removal – no incubation on ice

Features
High-throughput isolation of endotoxin-free plasmid DNA in 96-well format

  

• Plasmid DNA with less than 0.1 EU/μg for transfection of highly sensitive cells
• Patented endotoxin removal by additional washing step 
• Processing of up to 5 mL bacterial culture
• NucleoBond® 
Filter Plate for convenient filtration of bacterial lysates
• Processing under vacuum or by centrifugation
• Innovative MN Wash Plate minimizes risk of cross-contamination
• NucleoBond® Finalizer Plate to speed up DNA precipitation

Technology

Anion-exchange chromatography

Format

96-well plates

Processing Manual
Lysate clarification

96-well filter plates

Sample material

1–5 mL E. coli culture

Vector size

< 15 kbp
< 300 kbp (without NucleoBond® Finalizer Plate)

Typical yield

2–4 μg (1.5 mL LB/TB in 96-well culture plates)
10–50 µg (5 mL LB/TB in glass tubes)

A260/A280

1.80–1.95

Elution volume

100–200 μL

Preparation time

120 min/plate

Binding capacity 50 µg
Applications

• Manual isolation of high-copy plasmids

• Typical downstream applications: transfection, cloning, sequencing, PCR, restriction analysis

  
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Ordering information

Product

Preps

Specification REF
NucleoBond® 96 Xtra EF

1 x 96

NucleoBond® Xtra EF Plate, NucleoBond® Finalizer Plate, NucleoBond® Filter Plate, Culture Plate, MN Wash Plate, Elution Plate U-bottom, Gas-permeable Foil, Self-adhering Foil, buffers, RNase A

740430.1

4 x 96

as above 740430.4
Product accessories

Pack of

Specification REF
NucleoVac 96 Vacuum Manifold

1

740681
NucleoVac Vacuum Regulator

1

for controlling of vacuum 740641

For ordering click here

  
     
  
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