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NucleoSpin® 8 / 96 RNA Blood

RNA from whole blood

Direct blood lysis –
no selective erythrocyte lysis

Features
Isolation of total RNA from whole blood (human or animal) – flexible 8-well strip format for varying throughput or proven 96-well format for high throughput

• Direct total blood lysis – very simple and convenient procedure 
   (patent pending)
• No selective erythrocyte lysis at 4 °C – complete processing at RT
• Compatible with common blood collection tubes and anticoagulants 
  (e.g., EDTA, citrate, and heparin)
• Suitable for fresh and frozen blood samples
• rDNase included for efficient removal of genomic DNA
• Processing under vacuum or by centrifugation
• Suitable for manual and automated processing
• Innovative MN Wash Plate minimizes risk of cross-contamination

NucleoSpin® 8 RNA Blood

NucleoSpin® 96 RNA Blood

Technology Silica-membrane technology
Format 8-well strips

96-well plates

Processing Manual or automated, vacuum or centrifugation
Sample material < 400 µL whole blood (fresh or frozen)
Fragment size > 200 nt

> 200 nt

Typical yield 1–8 µg (400 µL blood)*

1–8 µg (400 µL blood)*

Typical RNA quality (A260/A280) 1.9–2.1

1.9–2.1

Elution volume

50–130 µL

50–130 µL

Preparation time

60 min/6 strips

100 min/plate

Binding capacity 100 µg

100 µg

* RNA yield strongly depends on the leukocyte number in each individual blood sample.

Applications*

• Manual or automated isolation of total RNA from fresh or frozen whole blood stabilized with, for example,
   EDTA, citrate, or heparin
• Typical downstream applications: qRT-PCR, next generation sequencing, blotting, array technology

* Kits to be used for research purposes only
  
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Ordering information

Product

Preps

Specification REF
NucleoSpin® 8 RNA Blood

12 x 8

NucleoSpin® RNA Blood Binding Strips, MN Wash Plates, Square-well Blocks, Racks of Tube Strips, Elution Plates U-bottom, Self-adhering Foil, buffers, RNase-free rDNase, Liquid Proteinase K 740220

60 x 8

as above 740220.5
NucleoSpin® 96 RNA Blood

2 x 96

NucleoSpin® RNA Blood Binding Plates, MN Wash Plates, Square-well Blocks, Round-well Blocks Low, Elution Plates U-bottom, Self-adhering Foil, buffers, RNase-free rDNase, Liquid Proteinase K 740225.2

4 x 96

as above 740225.4
Product accessories

Pack of

Specification REF
NucleoVac 96 Vacuum Manifold

1


740681
NucleoVac Vacuum Regulator

1

for controlling of vacuum 740641
Starter Set A

1

for use of NucleoSpin® 8-well strips on the NucleoVac 96 Vacuum Manifold 740682
Starter Set C

1

for use of NucleoSpin® 8-well strips under centrifugation 740684
MN Square-well Block

4

96-well blocks with 2.1 mL square wells 740476

24

as above 740476.24

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