User manual
NucleoSpin® RNA Blood Midi

Product flyer
NucleoSpin® RNA Blood
NucleoSpin® RNA Blood Midi

RNA from whole blood

Direct blood lysis –
no selective erythrocyte lysis
Midi spin kit for the isolation of RNA from fresh or frozen blood (human or animal)

• Direct total blood lysis – very simple and convenient procedure (patent pending)
• No selective erythrocyte lysis at 4 °C – complete processing at room temperature
• Superior RNA yield and quality from up to 1.3 mL whole blood
• Efficient on-column DNA removal for an increased sensitivity in downstream applications
• Compatible with common blood collection tubes and anticoagulants
   (e.g., EDTA, citrate, and heparin)
Technology Silica-membrane technology
Format Midi spin columns
Sample material

400–1300 µL whole blood (fresh or frozen)

Fragment size > 200 nt
Typical yield

4–26 µg (1300 µL whole blood)*

Typical yield (A260/A280) 1.9–2.1
Elution volume 200–400 µL
Preparation time 75 min/6 preps
Binding capacity 700 µg
* RNA yield strongly depends on the leukocyte number in each individual blood sample.


• Isolation of RNA from fresh or frozen whole blood from common blood collection tubes
   (EDTA, citrate, heparin)
• Typical downstream applications: qRT-PCR, next generation sequencing, blotting, array technology
* Kits to be used for research purposes only


Ordering information



Specification REF
NucleoSpin® RNA Blood Midi


NucleoSpin® RNA Blood Midi Columns with Collection Tubes, Collection Tubes (15 mL), buffers, RNase-free rDNase, Liquid Proteinase K 740210.20

For ordering click here

Copyright 2019 by MACHEREY-NAGEL GmbH & Co. KG   Imprint / Impressum Privacy StatementTerms Of Use