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NucleoBond® Xtra BAC

  
     

NucleoBond® Xtra BAC

Transfection-grade plasmid DNA

Features
The 2nd generation of anion exchanger for large construct plasmid DNA

  
• Optimized column design – one prep in approximately 75 min
• Column filter included – parallel lysate clearing and loading onto the column
• Optimal silica material – yields up to 150 μg
• Transfection-grade plasmid DNA using proven anion-exchange technology
• LyseControl for visualization of a completed lysis / neutralization

Technology Anion-exchange chromatography
Format Gravity flow columns
Lysate clarification Column filters
Sample material 250–750 mL E. coli culture
Vector size < 300 kbp
Typical yield 10–150 µg
A260/A280 1.80–1.95
Preparation time 75 min/2–4 preps
Binding capacity 150 µg
Applications

• Purification of P1, BACs, PACs, and other large constructs from E. coli
• Typical downstream applications: cloning, PCR, restriction analysis, sequencing, and transfection

 

  
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Ordering information

Product

Preps

Specification REF
NucleoBond® Xtra BAC

10

NucleoBond® Xtra BAC Columns with inserted NucleoBond® Xtra Column Filters, buffers, RNase A 740436.10

25

as above 740436.25
Product accessories

Pack of

Specification REF
NucleoBond® Xtra Combi Rack

1

for use with NucleoBond® Xtra Midi, Xtra Maxi, Xtra BAC, AX 20, AX 100, AX 500, AX 2000, and AX 10000 Columns 740415
NucleoBond® Smart Rack

1

for use with NucleoBond® Xtra Midi, Xtra Maxi, Xtra BAC, AX 20, AX 100, AX 500, AX 2000, and AX 10000 Columns 740413
NucleoBond® Xtra BAC Buffer Set

1

1000 mL of additional lysis buffers RES-BAC, LYS-BAC, NEU-BAC, and RNase A 740437

 

For ordering click here

  
     

NEWS

NucleoBond®
Xtra
Midi / Maxi

LyseControl
now included

 
  
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