User manual
Protino® Ni-NTA 

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Protein Purification Guide

Protino® Adaptor Sets


Protino® Ni-NTA Columns 1 mL/5 mL

Purification of His-tag proteins

Protino® Ni-NTA, the cost-effective NTA products for His-tag protein purification
• Chelating group NTA (nitrilotriacetic acid), two protein binding sites (also see Technologies)
• High capacity and high affinity
• Simply replace your current Ni-NTA products, no optimization or protocol change necessary
• Purification under native and denaturing conditions
• Suitable for small proteins, large protein complexes, proteins with low expression rates – universal use
• Market-leading performance for an unbeatable price

Protino® Ni-NTA Columns
• Ready-to-use FPLC™ columns filled with Protino® Ni-NTA Agarose

Protino® Ni-NTA Columns 1 mL Protino® Ni-NTA Columns 5 mL
Technology IMAC (immobilized metal ion affinity chromatography) IMAC (immobilized metal ion affinity chromatography)
Chelating ligand NTA (nitrilotriacetic acid) NTA (nitrilotriacetic acid)
Format 1 mL FPLC™ columns 5 mL FPLC™ columns
Column dimensions 0.7 cm (inner diameter) x 2.5 cm (height) 1.6 cm (inner diameter) x 2.5 cm (height)
Column body material Polypropylen Polypropylen
Column inlet port 10-32 (1/16'') female 10-32 (1/16'') female
Column outlet port 10-32 (1/16'') male 10-32 (1/16'') male
Matrix 6 % beaded agarose (cross-linked), precharged with Ni2+ 6 % beaded agarose (cross-linked), precharged with Ni2+
Bead size 45–165 µm 45–165 µm

Binding capacity*

 50 mg 250 mg 
Recommended flow rate**  1 mL/min 5 mL/min
Max. recommended flow rate 4 mL/min 10 mL/min
Storage solution 30 vol % ethanol 30 vol % ethanol
Storage temperature 4–8 °C 4–8 °C

* Binding capacity will vary for each polyhistidine-tagged protein
High flow rates may reduce binding capacity


• Purification of polyhistidine-tagged proteins
• Compatible with automated liquid chromatography systems (MPLC, FPLC™, ÄKTAdesign™, etc.),
   peristaltic pump, or syringe (adaptors may be necessary, please contact Technical Service)



Ordering information



Specification REF
Protino® Ni-NTA Columns 1 mL


FPLC™ columns 745410.5
Protino® Ni-NTA Columns 5 mL


FPLC™ columns 745415.1


as above 745415.5

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