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Protino® Ni-NTA 
Agarose

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Protein Purification Guide

  
     

Protino® Ni-NTA Agarose

Purification of His-tag proteins

 
Features
Protino® Ni-NTA, the cost-effective NTA products for His-tag protein purification

• Chelating group NTA (nitrilotriacetic acid), two protein binding sites (also see Technologies)
• High capacity and high affinity
• Simply replace your current Ni-NTA products, no optimization or protocol change necessary
• Purification under native and denaturing conditions
• Suitable for small proteins, large protein complexes, proteins with low expression rates 
   – universal use
• Market-leading performance for an unbeatable price

Protino® Ni-NTA Agarose
• Aqueous suspension
• For batch binding, gravity flow, and FPLC™ – highest flexibility of applications
• Empty Protino® Columns for gravity flow available

Technology

IMAC (immobilized metal ion affinity chromatography)

Chelating ligand NTA (nitrilotriacetic acid)
Format

50 % (v/v) aqueous suspension containing 30 % ethanol

Matrix

6 % beaded agarose (cross-linked), precharged with Ni2+

Bead size

45–165 µm

Binding capacity* Up to 50 mg/mL settled agarose
Storage temperature

4–8 °C

* Binding capacity will vary for each polyhistidine-tagged protein
  
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Ordering information

Product

Pack of

Specification REF
Protino® Ni-NTA Agarose

25 mL

settled beaded agarose

745400.25


100 mL

as above 745400.100

500 mL

as above 745400.500




Product accessories

Pack of

Specification REF
Protino® Columns 14 mL

10

empty columns (gravity flow)
for use with, e.g., Protino® Ni-NTA Agarose
745250.10
Protino® Columns 35 mL

10

empty columns (gravity flow)
for use with, e.g., Protino® Ni-NTA Agarose
745255.10

For ordering click here

  
   
  
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