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Protino® Ni-NTA Agarose
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Purification of His-tag proteins
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| Features |
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| Protino® Ni-NTA, the cost-effective NTA products for His-tag protein purification |
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• Chelating group NTA (nitrilotriacetic acid), two protein binding sites (also see Technologies)
• High capacity and high affinity
• Simply replace your current Ni-NTA products, no optimization or protocol change necessary
• Purification under native and denaturing conditions
• Suitable for small proteins, large protein complexes, proteins with low expression rates
– universal use
• Market-leading performance for an unbeatable price
Protino® Ni-NTA Agarose
• Aqueous suspension
• For batch binding, gravity flow, and FPLC™ – highest flexibility of applications
• Empty Protino® Columns for gravity flow available
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| Technology |
IMAC (immobilized metal ion affinity chromatography)
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| Chelating ligand |
NTA (nitrilotriacetic acid) |
| Format |
50 % (v/v) aqueous suspension containing 30 % ethanol
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| Matrix |
6 % beaded agarose (cross-linked), precharged with Ni2+
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Bead size
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45–165 µm
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| Binding capacity* |
Up to 50 mg/mL settled agarose |
| Storage temperature |
4–8 °C
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| * Binding capacity will vary for each polyhistidine-tagged protein |
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Ordering information
| Product |
Pack of
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Specification |
REF |
| Protino® Ni-NTA Agarose |
25 mL
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settled beaded agarose |
745400.25
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100 mL
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as above |
745400.100 |
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500 mL
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as above |
745400.500 |
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| Product accessories |
Pack of
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Specification |
REF |
| Protino® Columns 14 mL |
10
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empty columns (gravity flow)
for use with, e.g., Protino® Ni-NTA Agarose |
745250.10 |
| Protino® Columns 35 mL |
10
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empty columns (gravity flow)
for use with, e.g., Protino® Ni-NTA Agarose |
745255.10 |
For ordering click here
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