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NucleoSpin®
totalRNA FFPE XS


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RNA purification guide

  
     
NucleoSpin® totalRNA FFPE XS

Total RNA from FFPE samples

Improved yield with a new lysis chemistry
Features
Two-in-one kit for the isolation of small (e.g., miRNA) and large RNA from even very limited formalin-fixed, paraffin-embedded samples

 

 

• Blue colored Paraffin Dissolver (patented technology) included - very easy and convenient 
   paraffin removal
• rDNase included – efficient on-column DNA removal
• Higher sensitivity with concentrated RNA – elution volume as little as 5 µL
• High quality RNA for enhanced RT-PCR performance
Technology Silica-membrane technology
Format Mini spin columns-XS design
Sample material

1–3 sections (á 10 μm, á up to 6 cm2 tissue area), < 5 mg of tissue

Typical yield Depending on amount and quality of the sample
Elution volume RNA 5–30 µL
Preparation time 70 min/6 preps (90 min including optional rDNase digest)
Binding capacity 100 µg

Applications*

• Rapid isolation of RNA from formalin-fixed, paraffin-embedded samples, e.g., colon carcinoma (unstained),
   colon carcinoma (hematoxylin stained), colon mucosa, adenocarcinoma of colon transversum, liver
   (rat/human), liver carcinoma (hematoxylin stained), lymph node, spleen (rat/human), pancreas
   (rat/human; diabetic/non-diabetic), placenta
• Isolation of RNA from fresh and archived FFPE samples
• Isolation of RNA from specimen on object slides (stained or unstained)
• Typical downstream application: RT-PCR

Kits to be used for research purposes only
  
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Ordering information

Product

Preps

Specification REF
NucleoSpin® totalRNA FFPE XS

10

NucleoSpin® totalRNA FFPE XS Columns, Collection Tubes (2 mL), Collection Tubes (1.5 mL), Paraffin Dissolver, buffers, Proteinase K, RNase-free rDNase

740969.10

50

as above 740969.50

250

as above 740969.250

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