Editors
     
03/18
 Topic
"High Definition" in TLC?



We know the term "high definition" for high-definition television. But what is the meaning of it in the analytical method thin-layer chromatography (TLC)?
  The term "high definition"
  "High definition" is mostly used in connection with High Definition Television (HDTV). HDTV refers to a number of television standards that are distinguished from Standard Definition Television (SDTV) by increased vertical, horizontal or temporal resolution [1]. In addition to the resolution, other properties have improved, such as the color representation through extended color space coverage.

  TLC plates with high definition characteristics
  In thin-layer chromatography, sharp bands, good visualization of the separated spots and sufficiently high Rf values are important in order to obtain the needed results for good analyte identification and quantification.

The new SIL HD glass plates fulfill all requirements of modern thin-layer chromatography. High-resolution properties, outstanding staining characteristics and low-noise background of the silica gel layer as well as an UV indicator with brilliant luminosity.

The following separation of vitamins shows the high luminosity of the SIL HD UV254 compared to a competitive plate of the same size.
 


  Figure 1: Separation of vitamins (Appl. No. 403800)


  At a detection wavelength of 366 nm, the SIL HD convinces with excellent visibility of the bands.

 


  Figure 2: Separation of aflatoxins with detection at 366 nm (Appl. No. 403740)


  Application No. 403750 of the MN application database shows the separation of aflatoxins on the HPTLC plate Nano-SIL HD. The narrow particle size distribution of the HPTLC silica gel particles achieves even higher resolution with shorter migration distances and times compared to standard silica gel layers.

An optimized binder system provides good abrasion resistance and excellent dyeability of the silica gel 60 layer. The example of the steroids separation shows the plate successfully stained with a 100 % aqueous potassium permanganate solution.

 


  Figure 3: Separation of steroids with permanganate staining (Appl. No. 403810)


  Conclusion
  Although it is not possible to watch TV with TLC plates, SIL HD and Nano-SIL HD guarantee perfect results in thin-layer chromatography.


  Literature
  [1] https://de.wikipedia.org/wiki/High_Definition (24.05.2018)
 
Image credits
© Jag_cz · cirquedesprit · rr145 – stock.adobe.com
 
Contact the author – Please click here!
 
  More informationen – PDF download of SIL HD and Nano-SIL HD flyer
 

 Print   
 MN Special Offer

Free Samples of SIL HD and Nano-SIL HD glass plates for TLC


Try our high quality TLC plates for precise colorization in TLC!

These “High Definition” TLC and HPTLC glass plates are available with or without fluorescent indicator.


Sample pack of 3 glass plates:

SIL HD (REF 809213)
layer thickness: 0.25 mm, format: 20 x 20 cm, TLC

SIL HD UV254 (REF 809223)
layer thickness: 0.25 mm, format: 20 x 20 cm, with UV indicator, TLC


Nano-SIL HD (REF 811213)
layer thickness: 0.20 mm, format: 10 x 20 cm, HPTLC

Nano-SIL HD UV254 (REF 811223)
layer thickness: 0.20 mm, format: 10 x 20 cm, with UV indicator, HPTLC
 
  • High luminosity
  • Brilliant staining properties
  • Excellent separation efficiency

 
More INFORMATION about SIL HD – Please click here!

More INFORMATION about Nano-SIL HD – Please click here!


Order your free personal samples today!
Just contact MN by referring to promotion code “HD” and we will arrange all necessity with your local MN distributor.


To take advantage of this SPECIAL OFFER – Please click here!

 MN Application Highlight

Analysis of pesticides in tap water with a HLB (SPE) and
a core-shell phase (HPLC-MS/MS)



Drinking water is the most important food. Thus, a strict control is necessary for the observance of the tap water quality. For a comprehensive analysis of pesticides from various chemical classes and their metabolites, phases with a broad selectivity are needed.

Due to their wide application range "hydrophilic-lipophilic balanced" (short: "HLB") solid phases are successful for a reliable extraction from tap water. The presented SPE application shows the enrichment of various pesticides and metabolites by CHROMABOND® HLB with good and reproducible recovery rates.

The HPLC determination succeeded on the efficient core-shell phase NUCLEOSHELL® PFP – a hydrophobic pentafluorophenyl phase with distinctly expanded polarity in comparison to C18 phases.


View FULL SPE APPLICATION MN Appl. No. 306360 – Please click here!

View FULL HPLC APPLICATION MN Appl. No. 128150 – Please click here!

You can find more than 4000 further applications in the MN application database!


 MN Product Highlight

Traceability of samples in the lab by barcode
Ready-to-use solution: MN barcode-labeled vials
  A clear identification of samples and their traceability in all steps of sample preparation up to the finished analysis are nowadays essential requirements when working in the laboratory.
Barcoded vials are an efficient solution because they can be read quickly. The frequency of
errors caused by manual entries can be significantly reduced by barcode-labeled vials, thus
making the workflow safer. Especially with high sample throughput and progressive
automation, the automatic data transfer via barcode is an essential component for efficient
and cost-saving data management.
  For this reason, MACHEREY-NAGEL offers as an additional service both 1-D and 2-D barcode labeling for all 1.5 mL vials and larger. Individual customer requirements can be considered in the design of the label and its contents. These include, for example, the choice between ladder setup or fence setup for the 1-D barcode, additional clear text of the barcode and possibly additionally printed text (if space permits), font and barcode size, etc.

1-D barcode
2-D barcode

  In comparison to the 1-D barcode, the 2-D barcode has the advantage that a considerably larger amount of data can be encrypted on a small area. In addition, the ECC 200 symbol uses a very powerful error correction, which makes the code even in case of partial destruction still readable.

The labels used are characterized by good chemical resistance and excellent adhesion to glass.

 


If you are interested in barcode-labeled vials, please do not hesitate
to contact us at vials@mn-net.com!


 MN Literature Recommendation

Adsorbents for column chromatography
A brief overview


 

In this brochure you will find an overview about the adsorbents for column chromatography available from MACHEREY-NAGEL. Furthermore details of the following important adsorbents are presented:


  • Polyamide 6
  • Standard silica
 
More
INFORMATION about our adsorbents for column chromatography!


Order a HARDCOPY – Please click here!

DOWNLOAD of PDF version!
 


 Contact MN

Subscription / International contact


Not yet listed for the next MN Chroma-News  … four annual interesting updates in chromatography?
To SUBSCRIBE for MN Chroma-News – Please click here!


List of all international MN DISTRIBUTORS and subsidiaries - Please click here! 
They will be glad to help you with your questions concerning our chromatography products.


Any feedback, ideas, questions? … please contact us directly at info@mn-net.com





Copyright 2018 by MACHEREY-NAGEL GmbH & Co. KG   Imprint / Impressum Privacy StatementTerms Of Use