User manual
NucleoSpin® RNA Clean-up XS

NucleoSpin® RNA Clean-up XS

RNA clean-up

Easy and fast concentration

Highly efficient clean-up and concentration of RNA samples

• Complete removal of RT-PCR inhibitors
Very high RNA recovery and concentration
• RNA clean-up from pre-purified RNA (phenol / chloroform), enzymatic reactions
   (e.g., amplification reactions, labeling reactions, DNase digestions)
• Time-saving procedure based on NucleoSpin® RNA XS technology
• Input volume up to 300 μL
• Elution in as little as 5 μL
Technology Silica-membrane technology
Format Mini spin columns – XS design
Sample material < 300 µL RNA solution containing < 90 µg RNA
Fragment size > 200 nt
Typical recovery 85–95%
A260/A280 1.9–2.1
Typical RIN (RNA integrity number) Depending on sample quality (no significant loss of RIN detected)
Elution volume 5–30 µL
Preparation time 20 min/6 preps
Binding capacity 110 µg


RNA clean-up of
• Pre-purified RNA (e.g., with TRIzol®)
• DNase digestions (e.g., with MACHEREY-NAGEL rDNase Set)
• Reaction mixtures
• Up to 55-fold increase in concentration
• Typical downstream applications: enzymatic labeling reactions, RT-PCR, DNA/RNA-based chip hybridization

* Kits to be used for research purposes only



Ordering information



Specification REF
NucleoSpin® RNA Clean-up XS


NucleoSpin® RNA Clean-up XS Columns with Collection Tubes, Collection Tubes (2 mL), Collection Tubes (1.5 mL), buffers



as above 740903.50


as above 740903.250

Product accessories

Pack of

Specification REF
rDNase Set
click for detailed information


recombinant DNase and Reaction Buffer for rDNase, sufficient for 50 Mini preps of total RNA 740963

For ordering click here

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