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NucleoSpin® 8/96 Plasmid
NucleoSpin® 8/96 Plasmid Core Kit

Sequencing-grade plasmid DNA

Features

Isolation of plasmid DNA –flexible 8-well strip format for varying throughput and proven 96-well plate format for high troughput
• Time-saving parallel isolation of plasmid DNA 
Processing of up to 5 mL bacterial culture
• NucleoSpin® 8 Filter Strips/96 Filter Plate for convenient filtration of bacterial lysates
• Processing under vacuum or by centrifugation
• Suitable for manual or automated processing under vacuum
• Innovative MN Wash Plate minimizes risk of cross-contamination
• NucleoSpin® 8 / 96 Plasmid Core Kits: Kits with basic content focusing on
   automation platforms. Additional accessories can be combined as needed.

NucleoSpin® 8 Plasmid
NucleoSpin® 8 Plasmid Core Kit

NucleoSpin® 96 Plasmid
NucleoSpin® 96 Plasmid Core Kit

Technology

Silica-membrane technology

Format

8-well strips

96-well plates

Processing

Manual and automated, vacuum

Manual and automated, vacuum or centrifugation

Lysate clarification

8-well filter strips

96-well filter plates

Sample material

1–5 mL (1 mL E. coli culture, high copy) 

1–5 mL (1 mL E. coli culture, high copy)

Vector size

< 15 kbp

< 15 kbp

Typical yield

4–6 µg/mL E. coli culture

4–6 µg/mL E. coli culture

A260/A280

1.70–1.85

1.70–1.85

Elution volume

75–150 µL

75–150 µL

Preparation time

45 min/6 strips

45 min/plate

Binding capacity

20 µg

20 µg

Applications

• Manual and automated isolation of high-copy plasmids
• Support protocol available for purification of cosmids
• Typical downstream applications: cloning, sequencing, PCR, transformation, restriction analysis

* The NucleoSpin® 8/96 Plasmid Core Kits provide the buffers, RNase A, NucleoSpin® Plasmid Filter Strips/Plate, and NucleoSpin® Plasmid Binding Strips/Plate only. Accessory components (e.g., culture plate, elution plate, MN Wash Plate, and Wash Buffer AW) are not provided in the core kits but can individually be selected from a variety of suitable accessories (see ordering information). This allows highest flexibility for the user.
  
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Ordering information

Product

Preps

Specification REF

NucleoSpin® 8 Plasmid

12 x 8

NucleoSpin® Plasmid Filter Strips, NucleoSpin® Plasmid Binding Strips, Culture Plate, MN Wash Plate, Rack of Tube Strips, buffers, RNase A 740621

60 x 8

as above 740621.5
NucleoSpin® 8 Plasmid Core Kit

48 x 8

NucleoSpin® Plasmid Filter Strips, NucleoSpin® Plasmid Binding Strips, buffers, RNase A 740461.4
NucleoSpin® 96 Plasmid

1 x 96

NucleoSpin® Plasmid Filter Plate, NucleoSpin® Plasmid Binding Plate, MN Wash Plate, MN Square-well Block, Elution Plate U-bottom, Gas-permeable Foil, Self-adhering Foil, buffers, RNase A

740625.1

4 x 96

as above 740625.4
24 x 96 as above 740625.24
NucleoSpin® 96 Plasmid Core Kit

4 x 96

NucleoSpin® Plasmid Filter Plates, NucleoSpin® Plasmid Binding Plates, buffers, RNase A

740616.4
24 x 96 as above 740616.24
Product accessories

Pack of

Specification REF
NucleoVac 96 Vacuum Manifold

1

740681
NucleoVac Vacuum Regulator

1

for controlling of vacuum 740641
Starter Set A

1

for use of NucleoSpin® 8-well strips on the NucleoVac 96 Vacuum Manifold 740682
Culture Plate

4 sets

Square-well Blocks with Gas-permeable Foil 740488

24 sets

as above 740488.24
MN Square-well Block

4

96-well blocks with 2.1 mL square wells 740476

24

as above 740476.24
Round-well Block with Cap Strips

4 sets

1 set consists of 1 Round-well Block and 12 Cap Strips 740475

24 sets

as above 740475.24
Rack of Tube Strips

4 sets

1 set consists of 1 rack, 12 strips with 8 tubes each, and 12 Cap Strips 740477

24 sets

as above 740477.24
Round-well Block Low

4 sets

96-well blocks with 0.8 mL round wells and Self-adhering Foil 740487

24 sets

as above 740487.24
Buffer AW

1 L

wash buffer 740916.1

For ordering click here

  
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