User manual
Protino® Glutathione Agarose 4B

Product flyer
Protein Purification Guide


Protino® Glutathione Agarose 4B

Purification of GST-tag proteins


Protino® Glutathione Agarose 4B, best choice for cost-effective GST-tag protein purification
• Highest performance equivalent to Glutathione Sepharose™ 4B / GSTrap™ 4B columns
• Simply replace your current products, no optimization or protocol change necessary
• Suitable for small proteins, large protein complexes, proteins with low expression rates 
   – universal use
• Market-leading performance for an unbeatable price

Protino® Glutathione Agarose 4B
• Aqueous suspension
• For batch binding, gravity flow, and FPLC™ – highest flexibility of applications
• Empty Protino® columns for gravity flow available
Technology Affinity chromatography
Chelating ligand Glutathione, linked via sulfur atom
Format 75 % (v/v) aqueous suspension containing 20 % ethanol


4 % beaded agarose
Bead size 90 µm
Binding capacity*

8 mg/mL

Recommended flow rates
1 mL bed volume (column with 6.6 mm inner diameter):
Sample loading**: 0.2–1.0 mL/min
Washing / elution: 1.0 mL/min
10 mL bed volume (column with 16 mm inner diameter):
Sample loading**: 0.5–5.0 mL/min
Washing / elution: 5.0 mL/min
Max. linear flow rate 250 cm/h
Chemical stability

Protino® Gutathione Agarose 4B withstands incubation in 0.1 M acetate pH 4,
0.1 M NaOH, 70 % ethanol, or 6 M guanidine hydrochloride without significant loss of protein yield

Storage temperature 4–8 °C
Note Do not autoclave the gel
*   Binding capacity will vary for each GST-tagged protein
** Slow flow rates are recommended for the loading step to allow maximal binding of the GST-tagged protein
• Purification of GST-tagged proteins
• Batch binding, gravity-flow column chromatography, MPLC / FPLC™ applications


Ordering information


Pack of

Specification REF
Protino® Glutathione Agarose 4B

10 mL

settled beaded agarose 745500.10

100 mL

as above 745500.100

For ordering click here

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