Protino Glutathione Agarose 4B

Protino® Glutathione Agarose 4B	Purification of GST-tag proteins

Features

Protino® Glutathione Agarose 4B, best choice for cost-effective GST-tag protein purification
• Highest performance equivalent to Glutathione Sepharose™ 4B / GSTrap™ 4B columns • Simply replace your current products, no optimization or protocol change necessary • Suitable for small proteins, large protein complexes, proteins with low expression rates – universal use • Market-leading performance for an unbeatable price Protino® Glutathione Agarose 4B • Aqueous suspension • For batch binding, gravity flow, and FPLC™ – highest flexibility of applications • Empty Protino® columns for gravity flow available

Technology

Affinity chromatography

Chelating ligand

Glutathione, linked via sulfur atom

Format

75 % (v/v) aqueous suspension containing 20 % ethanol

Matrix

4 % beaded agarose

Bead size

90 µm

Binding capacity*

8 mg/mL

Recommended flow rates

1 mL bed volume (column with 6.6 mm inner diameter):
Sample loading**:	0.2–1.0 mL/min
Washing / elution:	1.0 mL/min

10 mL bed volume (column with 16 mm inner diameter):
Sample loading**:	0.5–5.0 mL/min
Washing / elution:	5.0 mL/min

Max. linear flow rate

250 cm/h

Chemical stability

Protino® Gutathione Agarose 4B withstands incubation in 0.1 M acetate pH 4,
0.1 M NaOH, 70 % ethanol, or 6 M guanidine hydrochloride without significant loss of protein yield

Storage temperature

4–8 °C

Note

Do not autoclave the gel

* Binding capacity will vary for each GST-tagged protein
** Slow flow rates are recommended for the loading step to allow maximal binding of the GST-tagged protein

Applications

• Purification of GST-tagged proteins
• Batch binding, gravity-flow column chromatography, MPLC / FPLC™ applications