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Protino® Glutathione Agarose 4B
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Purification of GST-tag proteins
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Features
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Protino® Glutathione Agarose 4B, best choice for cost-effective GST-tag protein purification |
• Highest performance equivalent to Glutathione Sepharose™ 4B / GSTrap™ 4B columns
• Simply replace your current products, no optimization or protocol change necessary
• Suitable for small proteins, large protein complexes, proteins with low expression rates
– universal use
• Market-leading performance for an unbeatable price
Protino® Glutathione Agarose 4B
• Aqueous suspension
• For batch binding, gravity flow, and FPLC™ – highest flexibility of applications
• Empty Protino® columns for gravity flow available |
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Technology |
Affinity chromatography |
Chelating ligand |
Glutathione, linked via sulfur atom |
Format |
75 % (v/v) aqueous suspension containing 20 % ethanol |
Matrix
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4 % beaded agarose |
Bead size |
90 µm |
Binding capacity* |
8 mg/mL
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Recommended flow rates |
1 mL bed volume (column with 6.6 mm inner diameter): |
Sample loading**: |
0.2–1.0 mL/min |
Washing / elution: |
1.0 mL/min |
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10 mL bed volume (column with 16 mm inner diameter): |
Sample loading**: |
0.5–5.0 mL/min |
Washing / elution: |
5.0 mL/min |
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Max. linear flow rate |
250 cm/h |
Chemical stability |
Protino® Gutathione Agarose 4B withstands incubation in 0.1 M acetate pH 4,
0.1 M NaOH, 70 % ethanol, or 6 M guanidine hydrochloride without significant loss of protein yield
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Storage temperature |
4–8 °C |
Note |
Do not autoclave the gel |
* Binding capacity will vary for each GST-tagged protein
** Slow flow rates are recommended for the loading step to allow maximal binding of the GST-tagged protein |
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Applications |
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• Purification of GST-tagged proteins
• Batch binding, gravity-flow column chromatography, MPLC / FPLC™ applications |
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Ordering information
Product |
Pack of
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Specification |
REF |
Protino® Glutathione Agarose 4B |
10 mL
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settled beaded agarose |
745500.10 |
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100 mL
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as above |
745500.100 |
For ordering click here
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