User manual
NucleoSpin® RNA Plus

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RNA purification guide

NucleoSpin® RNA Plus

RNA from cells and tissue

  NucleoSpin® gDNA Removal Column included

New generation for efficient and quick RNA isolation from cells and tissues

• NucleoSpin® gDNA Removal Column – no time consuming rDNase digestion
• Convenient handling – lysate clearing and gDNA removal with one column in one step
• New efficient lysis buffer – no b-mercaptoethanol or TCEP necessary

Technology Two column silica-membrane system
1. column for DNA removal
2. column for RNA isolation
Format Mini spin columns
Sample material

< 1 x 107 cultured cells,
< 109 bacterial cells,
< 108 yeast cells,
< 30 mg human / animal tissue

Fragment size > 200 nt
Typical yield

HeLa cells 5 x 106: 40-60 µg
Mouse liver 20 mg: 80-100 µg
Mouse kidney 20 mg: 40-70 µg
Mouse spleen 5 mg: 30-60 µg

A260/A280 1.9–2.1
A260/A230 1.8-2.5
Typical RIN (RNA integrity number) > 9
Elution volume 30–120 µL
Preparation time 20 min/6 preps
Binding capacity 200 µg


• RNA isolation from cultured cells, animal tissue, plant tissue, microorganisms
• Typical downstream applications: real-time RT-PCR, Northern blotting, primer extension, array technology,
   RNase protection assays

* Kits to be used for research purposes only



Ordering information



Specification REF
NucleoSpin® RNA Plus


NucleoSpin® RNA Plus Columns with Collection Tubes, NucleoSpin® gDNA Removal Columns with Collection Tubes, Collection Tubes (2 mL), Collection Tubes (1.5 mL), buffers 740984.10


as above 740984.50


as above 740984.250

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