User manual 
NucleoSpin® miRNA

Supplementary protocols
NucleoSpin® miRNA

Product flyer
RNA purification guide

NucleoSpin® miRNA

Small and large RNA isolation

No toxic phenol / chloroform –
convenient extraction
Isolation of total RNA (small and large RNA) from diverse sample materials

• Excellent total RNA recovery and purity by chaotropic salt lysis without phenol/chloroform
   (patent pending)
• Support protocol for fractionation of RNA by size – small RNA (< 200 nt) and large RNA (> 200 nt)
   in two separate fractions
• Additional isolation of total protein fraction ready to use for SDS-PAGE and Western blot analysis
• NucleoSpin® Filters for efficient sample homogenization
• rDNase for efficient on-column removal of genomic DNA

Technology Silica-membrane technology
Format Mini spin columns
Sample material 

< 107 cultured cells, < 30 mg human / animal tissue
< 50 mg plant tissue, < 150 µL reaction mixture

Fragment size ³ 18 nt
Typical yield 100 µg total RNA (107 HeLa cells: 10 µg small RNA,  90 µg large RNA)
Elution volume 30–100 µL
Preparation time < 45 min/6 preps (total RNA)
< 35 min/6 preps (small RNA only)
Binding capacity 200 µg


• Total RNA from human / animal tissue and cultured cells, from plant tissue, and in combination with
   phenol / chloroform (e.g., TRIzol®) lysis
• Purification of siRNA and large dsRNA from DICER reactions
• Typical downstream applications: real-time RT-PCR, Northern blotting, chip hybridization
* Kits to be used for research purposes only


Ordering information



Specification REF
NucleoSpin® miRNA


NucleoSpin® RNA Columns, NucleoSpin® Protein Removal Columns, Collection Tubes (2 mL, 2 mL lid, 1.5 mL), NucleoSpin® Filters, buffers, RNase-free rDNase 740971.10


as above 740971.50


as above 740971.250
Product accessories Pack of Specification REF
NucleoSpin® miRNA Column/Buffer Set 1 set NucleoSpin® RNA Columns (50), Buffer MW1, Buffer MW2 740304

* Kits for 10/50/250 preps are sufficient for 10/50/250 preps of small and large RNA when isolated in one fraction and are sufficient for 5/25/125 preps of small and large RNA when isolated separately.

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