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Instruction leaflet:
NUCLEODUR®
RP columns


NUCLEODUR® C18 Gravity-SB
High purity silica phase for HPLC
Monomeric octadecyl modification with extensive endcapping
13 % C · USP L1
Properties
Key features:
Hydrophobic C18 phase with distinct polar selectivity, ideal for method development, better retention of early eluting substances
Excellent performance under highly aqueous conditions
Suitable for LC/MS due to low bleeding characteristics
Technical characteristics:
Monomeric octadecyl modification, extensive endcapping;
pore size 110 Å; available particle sizes 1.8 μm, 3 μm and 5 μm;
carbon content 13 %; pH stability 1–9
Recommended application:
Overall sophisticated analytical separations, especially for polar compounds, e.g., antibiotics, water-soluble vitamins, organic acids
For applications click here.


Standard program* of columns packed with NUCLEODUR® C18 Gravity-SB
Column system Particle size: 1.8 µm 3 µm 5 µm
Analytical EC columns (standard system, 2–4.6 mm)
Preparative VarioPrep columns (VP, ≥ 8 mm)    
  if you do not know our HPLC column systems, click the "i" for further information
* Please note! Most combinations of MN column types, available column dimensions and particle sizes can be custom-packed on request.

EC standard columns for analytical HPLC
Ordering information
Eluent in column acetonitrile – water
Length -> 30 mm 50 mm 75 mm 100 mm 125 mm 150 mm 250 mm
NUCLEODUR® C18 Gravity-SB, 1.8 µm
2 mm ID 760591.20 760593.20 760595.20 760596.20   760598.20  
3 mm ID 760591.30 760593.30   760596.30      
4 mm ID 760591.40 760593.40   760596.40      
4.6 mm ID 760591.46 760593.46   760596.46      
EC guard columns* 4 x 2 mm 761990.20   4 x 3 mm 761990.30  
NUCLEODUR® C18 Gravity-SB, 3 µm
2 mm ID   760603.20   760606.20 760607.20 760608.20 760609.20
3 mm ID   760603.30   760606.30 760607.30 760608.30 760609.30
4 mm ID   760603.40   760606.40 760607.40 760608.40 760609.40
4.6 mm ID   760603.46 760605.46 760606.46 760607.46 760608.46 760609.46
EC guard columns* 4 x 2 mm 761991.20   4 x 3 mm 761991.30  
NUCLEODUR® C18 Gravity-SB, 5 µm
2 mm ID   760613.20   760616.20 760617.20 760618.20 760619.20
3 mm ID   760613.30   760616.30 760617.30 760618.30 760619.30
4 mm ID   760613.40   760616.40 760617.40 760618.40 760619.40
4.6 mm ID   760613.46 760615.46 760616.46 760617.46 760618.46 760619.46
EC guard columns* 4 x 2 mm 761992.20   4 x 3 mm 761992.30  
Guard columns in packs of 3, EC columns in packs of 1

Guard columns for EC columns   EC columns with ID Use guard
column holder
      2 mm 3 mm 4 mm 4.6 mm
* Column Protection System EC guard column 4/2 4/3 4/3 4/3 REF 718966
Custom-packed EC columns with other dimensions are available on request.
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VarioPrep columns for preparative HPLC
Ordering information
Eluent in column acetonitrile – water
Length 10 mm ID 21 mm ID 32 mm ID 40 mm ID
NUCLEODUR® C18 Gravity-SB, 5 µm
50 mm 762350.100 762350.210    
125 mm 762351.100 762351.210    
150 mm       762352.400
250 mm 762353.100 762353.210 762353.320 762353.400
VP guard columns with NUCLEODUR® C18 Gravity-SB
  8 mm ID 16 mm ID 32 mm ID
10 mm* 762354.80 762354.160    
15 mm*     762355.320
* VP guard column Size Pack of for VP column required holder REF
VP 10/8 10 x 8 mm ID 2 10 mm ID 8 mm 718251
VP 10/16 10 x 16 mm ID 2 21 mm ID 16 mm 718256
VP 15/32 15 x 32 mm ID 1 32–40 mm ID 32 mm 718253
Custom-packed VP columns with other dimensions are available on request.
For details of the VarioPrep column system click here.
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