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| Substances: | | THC; THC-COOH; 11-Nor-Δ9-THC-Carboxylic Acid |
| Column: | | CHROMABOND® Drug II, 3 ml, 200 mg |
| Phase: | | CHROMABOND Drug II |
| MN catalog number: | | 730680 |
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| Sample pretreatment: | | To 5 ml urine 300 μl 10 M potassium hydroxide solution and internal standard (for GC-MS deuterium labeled 11-nor-Δ9-THC-carboxylic acid) are added. The sample is vortexed and then hydrolysed at 60 °C for 15 min. The sample is cooled and 200 μl glacial acetic acid and 2 ml 50 mM ammonium acetate solution are added. If necessary the sample pH is adjusted to 6.0 – 7.0. |
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| Conditions: | | Column conditioning: 2 ml methanol, followed by 2 ml distilled water – equilibrate column with 2 ml 50 mM ammonium acetate buffer
Sample application: slowly force or aspirate the sample through the column (1-2 ml per min)
Column washing: interferences are eluted with 10 ml methanol – water (1:1, v/v); the column is dried for 10 min at high vacuum; the column is further washed with 2 ml acetonitrile and dried for another 2 min
Elution: THC metabolites are eluted with 3 ml hexane – ethyl acetate – glacial acetic acid (75:25:1, v/v/v) |
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Detection
Subsequent analysis: | | For further analysis we recommend GC-MS on an OPTIMA® 5 MS (e.g. Cat. No. 726220.30) column after derivatisation with 50 μl Silyl-991 (Cat. No. 701480; BSTFA –TMCS 99:1) at 70°C / 20 min – inject 1-2 μl onto GC. |
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| Author(-s): | | SPE department |
| Source: | | MACHEREY-NAGEL; Germany; 2006 |
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| Keywords: | | drugs; THC; urine |
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Chromatogram:
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Recovery rates: 70 - 80%
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| Legend: | | |