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Optical Resolution by Liquid Chromatography on Immobilized Bovine Serum Albumin

Method:
Matrix
Application-No.:
HPLC
-
105720

Substances: warfarin
Column: EC 150/4 RESOLVOSIL® BSA-7
Phase: Resolvosil BSA-7
MN catalog number: 720046.40
Sample pretreatment:
Conditions: eluent: 0.05M phosphate buffer pH 6.79 + 3% 1-propanol
flow rate: 2ml/min
Detection
Subsequent analysis:
UV, 225nm, 0.02 AUFS

Author(-s): Allenmark, S.
Source: J. Liquid Chromatogr. 9 (1986) 425 - 442

Keywords: enantiomers; cumarins
Chromatogram:


Legend: Optical resolution of (±)-warfarin, sample 50μM, 10μl injected.



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