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| Substances: |
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glycan strands; murein |
| Column: |
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NUCLEOSIL® 300-5C18, 250 x 4.6mm ID |
| Phase: |
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Nucleosil 300-5 C18 |
| MN catalog number: |
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720065.46 |
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| Sample pretreatment: |
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| Conditions: |
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temperature 50°C
eluent: convex gradient from 5 to 11% acetonitrile in 100mM Na phosphate buffer pH 2 over 90min
flow rate 0.5ml/min |
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Detection
Subsequent analysis: |
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upper trace UV, 202nm, lower trace radioactivity of 31μl aliquots of 125μl fractions |
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| Author(-s): |
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Harz H. |
| Source: |
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Anal. Biochem. 190 (1990) 120 - 128 |
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| Keywords: |
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carbohydrates |
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Chromatogram:
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| Legend: |
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Fig. 2. Separation of amidase digest of 150μg 3HGlcNAc-murein of E. coli EH
3247, numbers above peaks indicate length in disaccharide units. |