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PAH from Water

Method:
Matrix
Application-No.:
SPE
water
301250

Substances: acenaphthene; acenaphthylene; anthracene; benzanthracene; benzofluoranthene; benzoperylene; benzopyrene; chrysene; dibenzanthracene; fluoranthene; fluorene; indenopyrene; naphthalene; phenanthrene; pyrene
Column: CHROMABOND® C18 PAH / 6 ml / 2000 mg
Phase: CHROMABOND C18 PAH
MN catalog number: 730166
Sample pretreatment: Add 10 ml Methanol to 1000 ml water sample
Conditions: Column conditioning:
1 column volume methanol, then 1 column volume dist. water

Sample application:
Aspirate 1000 ml water sample through the column (about 15 to 20 ml/min), then
dry column (stream of nitrogen or 24 h in a desiccator over P2O5)

Elution:
elute with 4 ml acetonitrile - benzene* (3:1, v/v) and evaporate or fill up to the
volume required

* alternatively toluene can be used

Author(-s): Macherey-Nagel
Source: LC Department

Keywords: PAH
Recovery rates:

Compound (each 50 ng/l)

Recovery [%]

Naphthalene

87

Acenaphthylene

89

Acenaphthene

90

Fluorene

82

Phenanthrene

85

Anthracene

90

Fluoranthene

89

Pyrene

89

Benz[a]anthracene

87

Chrysene

95

Benzo[b]fluoranthene

91

Benzo[k]fluoranthene

89

Benzo[a]pyrene

90

Dibenz[ah]anthracene

97

Benzo[ghi]perylene

91

Indeno[1,2,3-cd]pyrene

96






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