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| Analysis of human hemoglobin chains |
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Method:
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Matrix
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Application-No.:
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HPLC
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human blood
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108240
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| Substances: |
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Hem; beta-globin; alpha-globin; AgammaT-globin; Ggamma-globin; AgammaI-globin |
| Column: |
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EC 250/4 NUCLEOSIL® 300-5 C4 MPN |
| Phase: |
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NUCLEOSIL® C4 MPN |
| REF number: |
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720245.40
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| Conditions: |
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Eluent A: 20 % acetonitrile, 80 % water, 0.1 % trifluoroacetic acid (TFA)
Eluent B: 60 % acetonitrile, 40 % water, 0.1 % trifluoroacetic acid (TFA)
Gradient: linear 40–60 % B in 60 min
Flow rate: 1 mL/min
Injection volume: 10 μL
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| Detection: |
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UV, 220 nm |
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| Author(-s): |
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LC department |
| Source: |
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MACHEREY-NAGEL |
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| Keywords: |
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proteins; hemoglobin; human blood
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Chromatogram:
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| Legend: |
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Separation of a hemolyzate of human blood
1. Hem
2. beta-globin
3. alpha-globin
4. AgammaT-globin
5. Ggamma-globin
6. AgammaI-globin |
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MACHEREY-NAGEL does not warrant the correctness of the applications. |
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