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Preparative separation and analysis of the 3HAbbott-69992, an HIV antiinfective nucleoside, by ligand exchange HPLC

Method:
Matrix
Application-No.:
HPLC
-
105670

Substances: HIV antiinfective nucleoside
Column: VP 250/10 NUCLEOSIL® CHIRAL-1
Phase: NUCLEOSIL® CHIRAL-1
REF number: 715075.100
Conditions: Eluent: 5 % acetonitrile - 95 % 0.25 mM copper(II) acetate, pH adjusted to 5.94 with acetic acid
Flow rate: 5 ml/min
Detection: UV, 254nm

Author(-s): Thomas, S.B.
Source: J. Chromatogr. 586 (1992) 265 - 270

Keywords: enantiomers
Chromatogram:



Legend: Enantiomer separation of 3HA-69992 showing cutoff points for fraction collection during preparative runs.



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