| | |
| Substances: | | neurotensin fragment 1-8; neurotensin fragment 8-13; neurotensin; myoglobin |
| Column: | | VA 50/4.6 NUCLEOGEL® RP 4000-8 |
| Phase: | | Nucleogel RP |
| MN catalog number: | | |
| | | |
| Sample pretreatment: | | Rapid reversed phase separation of peptides |
| | | |
| Conditions: | | eluent A: 0.1% trifluoroacetic acid (TFA) in acetonitrile - water (1:99); eluent B: 0.1% TFA in acetonitrile - water (99:1), linear gradient 10 - 60% B in 2 min
flow rate 4ml/min |
| | | |
Detection
Subsequent analysis: | | UV, 220nm |
| | |
|
| Author(-s): | | MACHEREY-NAGEL |
| Source: | | LC department |
| | |
|
| Keywords: | | peptides; proteins |
| | | |
| | |
Chromatogram:
| | |
|
| | | |
| Legend: | | Separation of 1. neurotensin fragment 1-8, 2. neurotensin fragment 8-13, 3.
neurotensin, 4. myoglobin. |