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PAH from Water Containing Humic Acids

Method:
Matrix
Application-No.:
SPE
water
301260

Substances:pah; naphthaline; acenaphthylene; acenaphthene; fluorene; phenanthrene; anthracene; fluoranthene; pyrene; benzo[a]anthracene; chrysene; benzo[b]fluoranthene; benzo[k]fluoranthene; benzo[a]pyrene; dibenzo[a,h]anthracene; benzo[g,h,i]perylene; indeno[1,2,3-c,d]pyren
Column:CHROMABOND® NH2/C18, 6 ml, 500/1000 mg (glass)
Phase:CHROMABOND NH2/C18
MN catalog number:730620 G
Sample pretreatment:Add 25 ml 2-propanol to 500 ml water sample
Conditions:Column conditioning:
10 ml methylene chloride, 10 ml methanol, then 10 ml dist. water - 2-propanol
(9:1, v/v)

Sample application:
Aspirate 500 ml pretreated water sample through the column (about 5 ml/min)

Column washing:
2 ml dist. water - 2-propanol (9:1, v/v), then dry column (about 20 min, vacuum)

Elution:
elute with 4 x1 ml methylene chloride (let percolate first 1 ml into the
column packing without vacuum, then apply light vacuum), if necessary evaporate
in a stream of nitrogen and fill up with a suitable solvent
Detection
Subsequent analysis:

Author(-s):Macherey-Nagel
Source:LC Department

Keywords:pah
Chromatogram:

Recovery Rates:
                Compound
Recovery %
                Compound
Recovery %
                Naphthaline
90
                Benzo[a]anthracene
88
                Acenaphtylene
89
                Chrysene
95
                Acenaphthene
86
                Benzo[b]fluoranthene
93
                Fluorene
87
                Benzo[k]fluoranthene
88
                Phenantrene
87
                Benzo[a]pyrene
87
                Anthracene
89
                Dibenzo[a,h]anthracene
91
                Fluoranthene
90
                Benzo[g,h,i]perylene
90
                Pyrene
93
                Indeno[1,2,3-c,d]pyren
89

Legend:



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