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Separation of mono- and disaccharides

Method:
Matrix
Application-No.:
HPLC
test mixture
102430

Substances: sucrose; maltose; glucose; xylose; galactose; arabinose; mannose
Column: VA 300/7.8 NUCLEOGEL® Sugar Pb
Phase: Nucleogel Sugar Pb
MN catalog number: 719530
Sample pretreatment:
Conditions: eluent: deionized water
flow rate 0.4 ml/min
pressure 25 bar
temperature 80 °C
Detection
Subsequent analysis:
RI

Author(-s): Macherey-Nagel
Source: LC Department

Keywords: sugars
Chromatogram:



Legend: Sample: 1. sucrose, 2. maltose, 3. glucose, 4. xylose, 5. galactose, 6.
arabinose, 7. mannose, injection volume 20 μl.



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