| | |
| Substances: | | pyridinoline; deoxypyridinoline; pyridoxine |
| Column: | | CHROMABOND® Crosslinks, 3ml/300mg |
| Phase: | | CHROMABOND Crosslinks |
| MN catalog number: | | 730458 |
| | | |
| Sample pretreatment: | | Hydrolise 250 μl urine and 50 μl of int. standard (Pyridoxin) in 250 μl konz.
HCl at ca. 100-105 °C for 12-16 h. Then add 2.5 ml washing solution (n-Butanol
/ glacial acetic acid (80:20, v/v)). |
| | | |
| Conditions: | | Conditioning:
5 ml of washing solution
Sample application:
Slowly force or aspirate sample through the column. Discard the eluate.
Column washing:
with 15-25 ml of the washing solution.
Elution:
3-5 ml distilled water |
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Detection
Subsequent analysis: | | |
| | |
|
| Author(-s): | | Macherey-Nagel |
| Source: | | LC department |
| | |
|
| Keywords: | | crosslinks |
| | | |
| | |
Chromatogram:
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| Legend: | | |