Separation of organics acids

Method:	Matrix	Application-No.:
HPLC		113740

Substances:		orotic acid (2,4-dihydroxypyrimidine-6-carboxylic acid); citric acid; pyruvic acid; malic acid; succinic acid; lactic acid; fumaric acid; formic acid; acetic acid; pyroglutamic acid; propionic acid; iso-butyric acid; butyric acid
Column:		VA 300/7.7 NUCLEOGEL SUGAR 810 H
Phase:		NUCLEOGEL SUGAR 810 H
MN catalog number:		719574
Conditions:		Eluents: 3.5 mmol H2SO4 Temperature: 65 °C Injection vol.: 5 µl Flow rate: 0.6 ml/min
Detection:		UV 210 nm
Author(-s):		LC department
Source:		MACHEREY-NAGEL
Keywords:		organic acids
Chromatogram:
Legend:		1. Orotic acid (2,4-Dihydroxypyrimidine-6-carboxylic acid) 2. Citric acid 3. Pyruvic acid 4. Malic acid 5. Succinic acid 6. Lactic acid 7. Fumaric acid 8. Formic acid 9. Acetic acid 10. Pyroglutamic acid 11. Propionic acid 12. iso-Butyric acid 13. Butyric acid

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