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Direct liquid chromatographic separation of enantiomers on immobilized protein stationary phases:
VI. Optical resolution of a series of racemic barbiturates – studies of substituent and mobile phase effects


Method:
Matrix
Application-No.:
HPLC
-
105810




Substances:
benzonal
Column:
EC 150/4 RESOLVOSIL BSA-7
Phase:
RESOLVOSIL BSA-7
REF number:
720046.40









Conditions:
Eluent: 50 mM phosphate buffer, pH 7.6 + 6 % 1-propanol
Flow rate: 1.0 mL/min



Detection:
UV, 210 nm



Author(-s):
Allenmark, S.
Source:
J. Chromatogr. 436 (1988), 479–483



Keywords:
enantiomers; barbiturates; anticonvulsant






Chromatogram:








Legend:
Optical resolution of the racemic anticonvulsant benzonal



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