Editors

Reversed phase separations of proteins

Method:
Matrix
Application-No.:
HPLC
-
108250




Substances:
ribonuclease; insulin; cytochrome C; lysozyme; bovine serum albumin; myoglobin; ovalbumin
Column:
VA 50/4.6 NUCLEOGEL® RP 300-8
Phase:
NUCLEOGEL® RP
REF number:
719460









Conditions:
Eluent A: 0.1 % TFA in water
Eluent B: 0.1 % TFA in acetonitrile - water (95:5, v/v)
Gradient: linear from 20 - 60 % B in 22 min
Flow rate: 1.5 mL/min



Detection:
UV, 220 nm



Author(-s):
LC department
Source:
MACHEREY-NAGEL



Keywords:
proteins






Chromatogram:









Legend:
1. ribonuclease
2. insulin
3. cytochrome C
4. lysozyme
5. bovine serum albumin
6. myoglobin
7. ovalbumin



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