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| Reversed phase separations of proteins | Method: | Matrix | Application-No.: | HPLC | - | 108250 | | | | | Substances: | | ribonuclease; insulin; cytochrome c; lysozyme; bovine serum albumin; myoglobin; ovalbumin; ribonuclease a; cytochrome c; lysozyme; bovine serum albumin; myoglobin; ovalbumin | | Column: | | VA 50/4.6 NUCLEOGEL® RP 300-8 VA 50/4.6 NUCLEOGEL® RP 4000-8 | | Phase: | | Nucleogel RP | | MN catalog number: | | | | | | | | Sample pretreatment: | | Reversed phase separations of proteins | | | | | | Conditions: | | a) eluent A: 0.1% trifluoroacetic acid (TFA) in water; eluent B: 0.1% TFA in acetonitrile - water (95 : 5), linear gradient from 20 - 60% B in 22min flow rate 1.5ml/min
b) eluent A: 0.1% TFA in acetonitrile - water (5:95, v/v), eluent B: 0.1% TFA in acetonitrile - water (95:5, v/v), linear gradient 18 - 60% B in 60s flow rate 4ml/min | | | | | Detection Subsequent analysis: | | UV, 220nm | | | | | | Author(-s): | | MACHEREY-NAGEL | | Source: | | LC department | | | | | | Keywords: | | proteins | | | | | | | | Chromatogram: | | |


Separation A Separation B | | | | | | Legend: | | A) Separation of 1. ribonuclease, 2. insulin, 3. cytochrome c, 4. lysozyme, 5. bovine serum albumin, 6. myoglobin, 7. ovalbumin.
B) Rapid separation of 1. ribonuclease A, 2. cytochrome c, 3. lysozyme, 4. bovine serum albumin, 5. myoglobin, 6. ovalbumin, total protein 0.34 mg. | | www.mn-net.com |  | Concerning questions to literature applications please contact the cited author/s. Chemicals mentioned in applications are not manufactured or sold by MACHEREY-NAGEL. Applications mentioned in MACHEREY-NAGEL literature or databases are provided for informational purposes only. MACHEREY-NAGEL does not warrant that all applications have been tested in MACHEREY-NAGEL laboratories using MACHEREY-NAGEL products. MACHEREY-NAGEL does not warrant the correctness of the applications. |
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