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| Reversed phase separations of proteins |
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Method:
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Matrix
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Application-No.:
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HPLC
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-
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108250
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| Substances: |
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ribonuclease; insulin; cytochrome C; lysozyme; bovine serum albumin; myoglobin; ovalbumin |
| Column: |
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VA 50/4.6 NUCLEOGEL® RP 300-8
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| Phase: |
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NUCLEOGEL® RP |
| REF number: |
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719460
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| Conditions: |
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Eluent A: 0.1 % TFA in water
Eluent B: 0.1 % TFA in acetonitrile - water (95:5, v/v)
Gradient: linear from 20 - 60 % B in 22 min
Flow rate: 1.5 mL/min
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| Detection: |
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UV, 220 nm |
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| Author(-s): |
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LC department |
| Source: |
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MACHEREY-NAGEL |
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| Keywords: |
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proteins |
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Chromatogram:
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| Legend: |
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1. ribonuclease
2. insulin
3. cytochrome C
4. lysozyme
5. bovine serum albumin
6. myoglobin
7. ovalbumin
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| www.mn-net.com |
 |
Concerning questions to literature applications please contact the cited author/s. Chemicals mentioned in applications are not manufactured or sold by MACHEREY-NAGEL. Applications mentioned in MACHEREY-NAGEL literature or databases are provided for informational purposes only. MACHEREY-NAGEL does not warrant that all applications have been tested in MACHEREY-NAGEL laboratories using MACHEREY-NAGEL products.
MACHEREY-NAGEL does not warrant the correctness of the applications. |
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