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| Reversed phase separations of proteins |
Method: | Matrix | Application-No.: | HPLC | - | 108250 |
| | | | Substances: | | ribonuclease; insulin; cytochrome c; lysozyme; bovine serum albumin; myoglobin; ovalbumin; ribonuclease a; cytochrome c; lysozyme; bovine serum albumin; myoglobin; ovalbumin | | Column: | | VA 50/4.6 NUCLEOGEL® RP 300-8
VA 50/4.6 NUCLEOGEL® RP 4000-8 | | Phase: | | Nucleogel RP | | MN catalog number: | | | | | | | | Sample pretreatment: | | Reversed phase separations of proteins | | | | | | Conditions: | | a) eluent A: 0.1% trifluoroacetic acid (TFA) in water; eluent B: 0.1% TFA in acetonitrile - water (95 : 5), linear gradient from 20 - 60% B in 22min
flow rate 1.5ml/min
b) eluent A: 0.1% TFA in acetonitrile - water (5:95, v/v), eluent B: 0.1% TFA in acetonitrile - water (95:5, v/v), linear gradient 18 - 60% B in 60s
flow rate 4ml/min | | | | | Detection
Subsequent analysis: | | UV, 220nm | | | |
| | Author(-s): | | MACHEREY-NAGEL | | Source: | | LC department | | | |
| | Keywords: | | proteins | | | | | | | | Chromatogram:
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Separation A Separation B
| | | | | | Legend: | | A) Separation of 1. ribonuclease, 2. insulin, 3. cytochrome c, 4. lysozyme, 5.
bovine serum albumin, 6. myoglobin, 7. ovalbumin.
B) Rapid separation of 1. ribonuclease A, 2. cytochrome c, 3. lysozyme, 4.
bovine serum albumin, 5. myoglobin, 6. ovalbumin, total protein 0.34 mg. |
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