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Rapid separation of sugars

Method:
Matrix
Application-No.:
HPLC
test mixture
102480

Substances: fructose; glucose; saccharose; lactose; maltose
Column: NUCLEOSIL® Carbohydrate; 250mm x 4mm ID
Phase: Nucleosil Carbohydrate
MN catalog number: 720905.40
Sample pretreatment: Rapid separation of sugars
Conditions: eluent: ACN - H2O (79:21, v/v)
flow rate 2ml/min
pressure 65bar
temperature 25°C
Detection
Subsequent analysis:
RI

Author(-s): Macherey-Nagel
Source: LC Department

Keywords: sugars
Chromatogram:



Legend: Separation of 1. fructose, 2. glucose, 3. saccharose, 4. maltose, 5. lactose.



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