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Determination of total dopamine, R- and S-salsolinol in human plasma by cyclodextrin bonded-phase liquid chromatography with electrochemical detection

Method:
Matrix
Application-No.:
HPLC
plasma
105540

Substances: dopamine; R-salsolinol; S-salsolinol
Column: EC 200/4 NUCLEODEX® beta-OH
Phase: NUCLEODEX® beta-OH
REF number: 720124.40
Sample: Concentration: 0.02 ng/ml plasma
Conditions: Eluent: 1 % TEA (triethylamine), 2 % methanol and 250 mg of 1-octanesulfonic acid per liter of freshly distilled water. The pH of the solution was adjusted to 3.9 with acetic acid
Flow rate: 0.5 ml/min
Detection: Electrochemical detection (0.4 V)

Author(-s): Sällström-Baum, S.
Source: J. Chromatogr. B, 660 (1994) 235 - 241

Keywords: enantiomers
Chromatogram:



Legend: HPLC elution profiles of the authentic standard solution peaks:
DA = dopamine (tR = 10.6 min)
S = S-salsolinol (tR = 11.8 min)
R = R-salsolinol (tR = 14.1 min)



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