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| Separation of representative whey proteins |
Method: | Matrix | Application-No.: | HPLC | whey | 108270 |
| | | | Substances: | | carboanhydrase; lactalbumin-a; lactoglobulin-b | | Column: | | VA 50/4.6 NUCLEOGEL® SAX 1000-8 | | Phase: | | NUCLEOGEL SAX | | MN catalog number: | | | | | | | | Sample pretreatment: | | Separation of representative whey proteins | | | | | | Conditions: | | eluent A: 0.02M Tris HCl pH 7.0; eluent B: A + 0.5M Na acetate pH 7.0, linear gradient 0 - 50% B in 10 min, then 50 - 100% B in 20 min
flow rate 1ml/min | | | | | Detection
Subsequent analysis: | | UV, 280nm | | | |
| | Author(-s): | | MACHEREY-NAGEL | | Source: | | LC department | | | |
| | Keywords: | | proteins | | | | | | | | Chromatogram:
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| | | | | | Legend: | | Separation of whey proteins, 1. carboanhydrase, 2. a-lactalbumin, 3.
b-lactoglobulin B, 4. b-lactoglobulin A. |
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MACHEREY-NAGEL does not warrant the correctness of the applications. |
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