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Method: | Matrix | Application-No.: | SPE | serum | 302020 |
| | | | Substances: | | benzoylecgonine; amphetamine; codeine; morphine | | Column: | | CHROMABOND® Drug / 3 ml / 200 mg | | Phase: | | CHROMABOND Drug | | MN catalog number: | | 730168 | | | | | | Sample pretreatment: | | Add 1.4 ml of 0.1 mol KH2PO4 buffer (pH 6) to 0.1 ml of blood serum and
centrifuge | | | | | | Conditions: | | Column conditioning:
2 ml methanol, then 2 ml of the 0.1 mol KH2PO4 buffer (pH 6)
Sample application:
Slowly force or aspirate the clear supernatant from the sample pretreatment
through the column
Column washing:
2 ml 0.1 mol KH2PO4 buffer (pH 6), then 1 ml 0.1 mol acetic acid, then 2 ml
methanol; afterwards dry column by centrifugation (2 min., 4000 rpm.) and under
vacuum for 10 min.
Elution:
1.5 ml dichloromethane / 2-propanol / ammonia solution (25 %) (80:20:2, v/v/v)
Further analysis: For further analysis we recommend HPLC with the column
CC 250/2 NUCLEOSIL 100-5 C18 AB (Cat.-No. 721 663.20) as described in our
application 1740 'Analysis of morphine, benzoylecgonine, cocaine, codeine from
serum'
or
GC/MS after derivatisation with perfluoropropanic acid/perfluoropropanol, i.e.
with a OPTIMA 5 MS, L: 30 m, ID: 0.25 mm, FT: 0.25 m (Cat.-No. 726 220.30) | | | | | Detection
Subsequent analysis: | | | | | |
| | Author(-s): | | Weinmann, W. | | Source: | | Blutalkohol 35, p. 1-9, (1998) | | | |
| | Keywords: | | drugs | | | | | | | | Chromatogram:
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| www.mn-net.com |  | Concerning questions to literature applications please contact the cited author/s. Chemicals mentioned in applications are not manufactured or sold by MACHEREY-NAGEL. Applications mentioned in MACHEREY-NAGEL literature or databases are provided for informational purposes only. MACHEREY-NAGEL does not warrant that all applications have been tested in MACHEREY-NAGEL laboratories using MACHEREY-NAGEL products.
MACHEREY-NAGEL does not warrant the correctness of the applications. |
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