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Rapid separation of 16 PAHs according to EPA

Method:
Matrix
Application-No.:
HPLC
115030

Substances:naphthalene; acenaphthylene; acenaphthene; fluorene; phenantrene; anthracene; fluoranthene; pyrene; benz[a]anthracene; chrysene; benzo[b]fluoranthene; benzo[k]fluoranthen; benzo[a]pyrene; dibenz[ah]anthracene; benzo[ghi]perylene; indeno[1,2,3-cd]pyrene
Column:EC 50/4 NUCLEOSIL 100-5 C18 PAH 50 x 4 mm ID
Phase:NUCLEOSIL 100-5 C18 PAH
MN catalog number:
Sample pretreatment:
Conditions:Eluent A: Water, Eluent B: Acetonitrile
Gradient: from 55 to 100% B in 2.5 min; then 3.5 min at
100% B; finally in 0.1 min from 100 to 55% B
Flow rate: 1 ml/min
Pressure: 25 – 30 bar
Temperature: 25 °C
Detection
Subsequent analysis:
UV 260 nm

Author(-s):LC Department
Source:MACHEREY-NAGEL

Keywords:pah
Chromatogram:



Legend:1. Naphthalene
2. Acenaphthylene
3. Acenaphthene
4. Fluorene
5. Phenantrene
6. Anthracene
7. Fluoranthene
8. Pyrene
9. Benz[a]anthracene
10. Chrysene
11. Benzo[b]fluoranthene
12. Benzo[k]fluoranthen
13. Benzo[a]pyrene
14. Dibenz[ah]anthracene
15. Benzo[ghi]perylene
16. Indeno[1,2,3-cd]pyrene



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