Redakteure

Amino acids

Method:
Matrix
Application-No.:
TLC
-
400190




Substances:
aspartic acid; glutamic acid; lysine; arginine; asparagine; glutamine; histidine; glycine; proline; serine; threonine; laryl-OH; cysteine; alanine; methionine; tyrosine; tryptophan; leucine; phenylalanine; isoleucine; valine; laryl-NH2
Plate:
POLYGRAM® Polyamide 6
Phase:
Polyamide 6
REF number:
803013



Sample pretreatment:
(laryl chloride = lissamine rhodamine B sulphonyl chloride)



Conditions:
Eluent:
I. ethyl acetate / ethanol / ammonium hydroxide (20:5:1, v/v)
II. n-propanol / chloroform / 99.7 % acetic acid (80:20:5, v/v)



Detection:
fluorescence, 560/595 nm



Author(-s):
Metrione, R.
Source:
J. Chromatogr. 363 (1986) 337



Keywords:
amino acids






Rf values:








Legend:
With fluorescence detection a detection limit of 400 fM can be achieved. Laryl chloride is also suited for derivatization of terminal amino groups in peptides.



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