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| Ion exchange separations of protein standards |
Method: | Matrix | Application-No.: | HPLC | - | 108260 |
| | | | Substances: | | myoglobin; chymotrypsinogen-a a; cytochrome c; lysozyme; myoglobin; conalbumin; ovalbumin; trypsininhibitor; soybean | | Column: | | VA 50/4.6 NUCLEOGEL® SCX 1000-8 | | Phase: | | NUCLEOGEL SCX | | MN catalog number: | | | | | | | | Sample pretreatment: | | Ion exchange separations of protein standards | | | | | | Conditions: | | a) eluent A: 0.02M KH2PO4 pH 6.0; eluent B: A + 0.5M NaCl pH 6.0, linear gradient 0 - 100% B in 20min
flow rate 1ml/min
b) eluent A: 0.01M Tris HCl pH 8.0; eluent B: A + 0.35M NaCl pH 8.0, linear gradient 0 - 100% B in 20min
flow rate 1ml/min | | | | | Detection
Subsequent analysis: | | UV, 280nm | | | |
| | Author(-s): | | MACHEREY-NAGEL | | Source: | | LC department | | | |
| | Keywords: | | proteins | | | | | | | | Chromatogram:
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| | | | | | Legend: | | a) Cation exchange separation of protein standards, 1. myoglobin, 2.
a-chymotrypsinogen A, 3. cytochrome c, 4. lysozyme.
b) Anion exchange separation of protein standards, 1. myoglobin, 2. conalbumin,
3. ovalbumin, 4. trypsininhibitor soybean. |
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MACHEREY-NAGEL does not warrant the correctness of the applications. |
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