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| Ion exchange separation of protein standard on NUCLEOGEL® SCX |
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Method:
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Matrix
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Application-No.:
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HPLC
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-
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108261
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| Substances: |
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myoglobin; α-chymotrypsinogen A; cytochrome c; lysozyme |
| Column: |
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VA 50/4.6 NUCLEOGEL® SCX 1000-8 |
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NUCLEOGEL® SCX |
| REF number: |
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719475
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| Sample: |
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protein standard |
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| Conditions: |
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Eluent A: 0.02 mol/L KH2PO4, pH 6.0
Eluent B: A + 0.5 mol/L NaCl, pH 6.0
Gradient: linear, 0–100 % B in 20 min
Flow rate: 1 mL/min
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| Detection: |
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UV, 280 nm |
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| Author(-s): |
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HPLC department |
| Source: |
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MACHEREY-NAGEL |
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| Keywords: |
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proteins; cation ion exchange |
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Chromatogram:
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| Legend: |
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1 - myoglobin
2 - α-chymotrypsinogen A
3 - cytochrome c
4 - lysozyme |
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Concerning questions to literature applications please contact the cited author/s. Chemicals mentioned in applications are not manufactured or sold by MACHEREY-NAGEL. Applications mentioned in MACHEREY-NAGEL literature or databases are provided for informational purposes only. MACHEREY-NAGEL does not warrant that all applications have been tested in MACHEREY-NAGEL laboratories using MACHEREY-NAGEL products.
MACHEREY-NAGEL does not warrant the correctness of the applications. |
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